Anti-Steroidogenic Factor 1 antibody (ab79377)

Overview

  • Product nameAnti-Steroidogenic Factor 1 antibody
    See all Steroidogenic Factor 1 primary antibodies
  • Description
    Rabbit polyclonal to Steroidogenic Factor 1
  • SpecificityThis antibody detects endogenous levels of total Steroidogenic Factor 1 protein.
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized non phosphopeptide derived from human Steroidogenic Factor 1 around the phosphorylation site of serine 203 (Y-A-SP-P-P)

  • Positive control
    • Extracts from rat lung.

Properties

Applications

Our Abpromise guarantee covers the use of ab79377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 52 kDa.
ELISA 1/5000.

Target

  • FunctionTranscriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3.
  • Involvement in diseaseDefects in NR5A1 are a cause of 46,XY disorder of sex development (46,XY DSD) [MIM:612965]; also known as XY sex reversal with or without adrenal failure. A congenital condition in which development of chromosomal, gonadal, or antomic sex is atypical. 46,XY DSD is a disorder of gonadal (testicular) development, which may be complete or partial. The complete form includes streak gonads, normal mullerian structures, and normal female external genitalia. The partial form includes ambiguous external genitalia and partial development of mullerian and wolffian structures.
    Defects in NR5A1 are a cause of adrenocortical insufficiency without ovarian defect (ACIWOD) [MIM:184757]. ACIWOD is characterized by severe 'slackness' muscular hypotonia. There is decreased sodium, increased potassium and elevated ACTH.
    Defects in NR5A1 are the cause of premature ovarian failure type 7 (POF7) [MIM:612964]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR5 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Post-translational
    modifications
    Acetylation stimulates the transcriptional activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AD4BP antibody
    • Adrenal 4 binding protein antibody
    • Adrenal 4-binding protein antibody
    • ELP antibody
    • FTZ 1 antibody
    • FTZ1 antibody
    • FTZF 1 antibody
    • FTZF1 antibody
    • Fushi tarazu factor (Drosophila) homolog 1 antibody
    • Fushi tarazu factor homolog 1 antibody
    • NR5A1 antibody
    • Nuclear receptor AdBP4 antibody
    • Nuclear Receptor Subfamily 5 group A member 1 antibody
    • POF7 antibody
    • SF 1 antibody
    • SF-1 antibody
    • SF1 antibody
    • SPGF8 antibody
    • SRXY3 antibody
    • Steroid hormone receptor Ad4BP antibody
    • Steroidogenic factor 1 antibody
    • Steroidogenic factor 1 nuclear receptor antibody
    • STF 1 antibody
    • STF-1 antibody
    • STF1 antibody
    • STF1_HUMAN antibody
    see all

Anti-Steroidogenic Factor 1 antibody images

  • All lanes : Anti-Steroidogenic Factor 1 antibody (ab79377) at 1/500 dilution

    Lane 1 : Extracts from rat lung
    Lane 2 : Extracts from rat lung with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 52 kDa
    Observed band size : 52 kDa

References for Anti-Steroidogenic Factor 1 antibody (ab79377)

ab79377 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - nuclear (Embryonic Hypothalamic Cell Line)
Specification Embryonic Hypothalamic Cell Line
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 3 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step Real-time PCR
Positive control Flag Antibody (Sigma) was used in parallel to IP the flag-tagged SF-1 stably expressed in the cells. I measured recruitment to a validated, endogenous SF-1 binding site.
Negative control As a negative control, I used normal mouse IgG (Santa Cruz) in a parallel IP. Enrichment at a known SF-1 binding site was measured by qPCR. Additionally, enrichment of negative control regions of the genome was also measured for each ChIP (not shown).
Username

Dr. Emily Faivre

Verified customer

Submitted Oct 28 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"