General notesTissue: Stomach
Location: Lesser curvature.
Diagnosis: Adenocarcinoma, well differentiated.
Age: 45 years
Gross findings: Ulcerative lesion located at the lesser curvature. Gray in color. Hemorrhagic around the lesion. Wall is 1 cm thick, firm, dirty, and hemorrhagic. Ulceration measures 2 x 2 cm in diameter.
Microscopic findings: Biopsy shows malignant columnar cells arranged in glands. The malignant cells have clear cytoplasm with vacuoles. Nuclei have irregular nuclear membranes, cytoplasm is granular and vacuolated with large nucleoli. Many monstrous nuclei. The malignant cells have invaded the gastric wall and there are many lymphocytes. There is evidence of mucinous secretion.
Insoluble protein fraction.
Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Please note that 20µl of 5X Laemmli buffer were added per 100µl of tissue lysate, as a result the lysate may not be suitable for applications which require native protein in a non-denaturing buffer. The final Laemmli buffer concs are: 31.25mM Tris HCl, pH 6.8, 12.5% Glycerol, 1% SDS, 0.005% Bromophenol Blue, 25mM TCEP.
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Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
Lysate notesThis product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction.
Please find below a range of products that relate to ab44266:
Extraction 1: soluble protein fraction
ab44264 Human Stomach Tumor Tissue lysate
ab44263 Human Stomach Normal Tissue lysate (matched)
Extraction 2: insoluble protein fraction
ab44266 Human Stomach Tumor Tissue lysate
ab44265 Human Stomach Normal Tissue lysate (matched)