Anti-Stra6 antibody (ab77616)
Key features and details
- Goat polyclonal to Stra6
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Stra6 antibody
See all Stra6 primary antibodies -
Description
Goat polyclonal to Stra6 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide:
QAFRKAALTSAKAN
, corresponding to C terminal amino acids 653-665 of mouse (NP_033317.1) and rat (NP_001025095.1) Stra6 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 74 kDa).
A 1 hour primary incubation is recommended for this product. Please note a non-specific band of ~80kDa. |
Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 74 kDa). A 1 hour primary incubation is recommended for this product. Please note a non-specific band of ~80kDa. |
Target
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Function
May act as a high-affinity cell-surface receptor for the complex retinol-retinol binding protein (RBP/RBP4). Acts by removing retinol from RBP/RBP4 and transports it across the plasma membrane, where it can be metabolized. This mechanism does not depend on endocytosis. Binds to RBP/RBP4 with high affinity. Increases cellular retinol uptake from the retinol-RBP complex. -
Tissue specificity
Broad expression. In adult eye expressed in sclera, retina, retinal pigment epithelium, and trabecular meshwork but not in choroid and iris. -
Involvement in disease
Defects in STRA6 are the cause of microphthalmia syndromic type 9 (MCOPS9) [MIM:601186]; also called Matthew-Wood syndrome or Spear syndrome. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS9 is a rare clinical entity including as main characteristics anophthalmia or severe microphthalmia, and pulmonary hypoplasia or aplasia.
Note=Mutations in STRA6 may be a cause of isolated colobomatous microphthalmia, a disorder of the eye characterized by an abnormally small ocular globe. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 363071 Rat
- SwissProt: Q4QR83 Rat
- Unigene: 12735 Rat
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Alternative names
- MCOPCB8 antibody
- MCOPS9 antibody
- PP14296 antibody
see all
Images
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All lanes : Anti-Stra6 antibody (ab77616) at 1 µg/ml
Lane 1 : Rat testes tissue lysate
Lane 2 : Rat testes tissue lysate with Immunizing peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 74 kDa
Observed band size: 80-90 kDa why is the actual band size different from the predicted?Primary incubation 1 hour at room temperature. Detected by chemiluminescence.
Datasheets and documents
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Datasheet download
References (1)
ab77616 has been referenced in 1 publication.
- Huang R et al. Retinol-Binding Protein 4 Activates STRA6, Provoking Pancreatic ß-Cell Dysfunction in Type 2 Diabetes. Diabetes 70:449-463 (2021). PubMed: 33199363