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Streptavidin Alkaline Phosphatase (Ready-To-Use) (ab128984)

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Overview

  • Product nameStreptavidin Alkaline Phosphatase (Ready-To-Use)
  • Tested applicationsIHC-P more details
  • General notesThis reagent is intended to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody that reacts with the primary antibody, enzyme-labeled streptavidin, and substrate chromogen.

    Procedure:

    1. Deparaffinize and rehydrate tissue section.

    2. Wash 2 times in buffer.

    3. If required, incubate tissue in digestive enzyme (or appropriate pretreatment).

    4. Wash 4 times in buffer.

    5. (Optional) Place slide in Protein Block (ab128988) and incubate for 5-10 minutes at room temperature to block nonspecific background staining. NOTE: Do not exceed 10 minutes or there may be a reduction in desired stain.

    6. Rinse (Optional).

    7. Apply primary antibody and incubate according to manufacturer's protocol.

    8. Wash 4 times in buffer.

    9. Incubate slide with biotinylated secondary solution according to manufacturer’s protocol.

    10. Wash 4 times in buffer.

    11. Incubate slide with Streptavidin Alkaline Phosphatase solution for 10 minutes at room temperature.

    12. Rinse 4 times in buffer.

    13. Place slide in appropriate chromogenic substrate and incubate until desired reaction is achieved.

    14. Counterstain and coverslip.

    NOTE: This reagent is provided in bulk form for use with automated systems or in staining jars for repeated use. If the DIP method is employed, staining jar should be sealed and refrigerated between uses. Remove staining jars from the refrigerator 30 minutes prior to staining to allow reagents to come to room temperature. The specificity and sensitivity of antigen detection is dependent on the specific primary antibody used.

    IHC Protocol Summary

    • Properties

  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.2% Proclin
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab128984 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IHC-P IHC-P: Use at an assay dependent concentration.

    References for Streptavidin Alkaline Phosphatase (Ready-To-Use) (ab128984)

    ab128984 has not yet been referenced specifically in any publications.

    Publishing research using ab128984 ? Please let us know so that we can cite the reference in this datasheet

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    Inquiry: Hi there, I was wondering whether you can help. I have to stain mouse tissue using an unconjugated primary antibody (Anti-Mouse/Rat MULT1, Host - Armenian Hamster IgG) and a biotinylated secondary one (Anti-Armenian Hamster IgG Biotin, Host - Goa...

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    Thank you for your inquiry.

    I can suggest to use a strepavidin that is coupled to an enzyme, for example: ab128985 (Streptavidin Peroxidase (Ready-To-Use)) and combine this with a suitable chromogen, for example: ab94665 (DAB substrate kit)
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"