Anti-Streptolysin O (Hemolytic streptococcus) antibody (ab188539)
Key features and details
- Rabbit polyclonal to Streptolysin O (Hemolytic streptococcus)
- Suitable for: WB, Neutralising, IP
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Streptolysin O (Hemolytic streptococcus) antibody -
Description
Rabbit polyclonal to Streptolysin O (Hemolytic streptococcus) -
Host species
Rabbit -
Specificity
ab188539 reacts with Streptolysin O of Group A, C and G origins. -
Tested applications
Suitable for: WB, Neutralising, IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein (His-tag) corresponding to Streptolysin O (Hemolytic streptococcus). (from S. dysgalactiae subsp. equismilis H46A (group C hemolytic Streptococci) expressed and highly purified from E. coli).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.09% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
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Neutralising |
Use at an assay dependent concentration.
Neutralization of hemolysis by Streptolysin O (SLO). |
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 64 kDa. |
Neutralising
Use at an assay dependent concentration. Neutralization of hemolysis by Streptolysin O (SLO). |
IP
Use at an assay dependent concentration. |
Target
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Relevance
Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. The membrane-damaging activity is measured by hemolysis of red-blood cell. SLO is oxygen-sensitive and is easily inactivated in its presence but can be reactivated by thiol compounds , so it is also called thiol-activated cytolysin. SLO is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and their homology is over 98%. -
Alternative names
- Streptolysin O (Thiol-activated cytolysin) slo antibody
- Streptolysin O antibody
Images
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All lanes : Anti-Streptolysin O (Hemolytic streptococcus) antibody (ab188539) at 1/2000 dilution
Lane 1 : Medium only
Lane 2 : Culture supernatant of GAS strain Sa
Lane 3 : Culture supernatant of GCS strain H46A
Predicted band size: 64 kDaMost of Streptolysin O in culture medium of GAS strain Sa is proteolytically degraded, accounting for low activity level of Streptolysin O in the culture of this strain. Condensed sample is analyzed in the second Western Blot.
The two faint bands below Streptolysin O band are partially degraded Streptolysin O.
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All lanes : Anti-Streptolysin O (Hemolytic streptococcus) antibody (ab188539) at 1/2000 dilution
Lane 1 : Concentrated culture of GSA strain Sa
Lane 2 : Concentrated medium only
Predicted band size: 64 kDaCulture supernatant was approximately 1,000~2,000 fold concentrated from culture medium by ammonium fractionation and heat-precipitation.
The Streptolysin O of GSA strains is degraded by an exoprotease called streptococcal pyrogenic exotoxin B and both the concentration and size of Streptolysin O in culture medium is decreased from the native one.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab188539 has been referenced in 2 publications.
- Vita GM et al. Human Serum Albumin Binds Streptolysin O (SLO) Toxin Produced by Group A Streptococcus and Inhibits Its Cytotoxic and Hemolytic Effects. Front Immunol 11:507092 (2020). PubMed: 33363530
- Hancz D et al. Inhibition of Inflammasome-Dependent Interleukin 1ß Production by Streptococcal NAD(+)-Glycohydrolase: Evidence for Extracellular Activity. MBio 8:N/A (2017). PubMed: 28720729