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SDS-PAGE - Streptolysin protein (ab63978)
(enlarge)
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Product Name
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Streptolysin O (Hemolytic streptococcus) protein
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Product type
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Proteins and Peptides
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Peptide
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The product was highly purified from E.coli over-expressing Streptolysin O of Group C hemolytic streptococci.
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Specificity
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The specific activity is as high as 700,000 to ~1,000,000 hemolytic units (HU) /mg.
Definition of 1HU is the activation of 50% hemolysis on 3% sheep red blood cells.
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Tested applications
(see key)
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FuncS, SDS-PAGE
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Application notes
(see key)
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Recommended dilutions
SDS-PAGE: Use at an assay dependent dilution.
FuncS: Enzyme activity: Use at an assay dependent dilution.
Also useful as antigen for the measurement of anti-streptolysin 0 antibody (AS0) (diagnostic reagent) and as a reagent for membrane pore formation to introduce small to macromolecules into living cells.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Microbiology >> Toxin >> Bacterial Toxin >> Streptococcus Toxin
Microbiology >> Organism >> Bacteria >> Gram positive bacteria >> Streptococcus
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Relevance
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Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. The membrane-damaging activity is measured by hemolysis of red-blood cell. SLO is oxygen-sensitive and is easily inactivated in its presence but can be reactivated by thiol compounds , so it is also called thiol-activated cytolysin (2). SLO is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and their homology is over 98%.
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: None
Constituents: 50% Glycerol, PBS, 1mM DTT Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Purification notes
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Purity >98% SDS-PAGE.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
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Expression system
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E. coli
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Streptolysin O (Hemolytic streptococcus) protein is on this datasheet. But please do contact us if you would like any reassurance! |
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Streptolysin O (Hemolytic streptococcus) protein images:
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 SDS-PAGE - Streptolysin protein (ab63978)
ab63978 on SDS-PAGE.
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Search PubMed (MEDLINE) for references to Streptolysin O (Hemolytic streptococcus)
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