Anti-Substance P antibody [SP-DE4-21] (ab14184)

Overview

• Product nameAnti-Substance P antibody [SP-DE4-21]See all Substance P primary antibodies ...
• Description
  Mouse monoclonal [SP-DE4-21] to Substance P
• SpecificityThe antibody reacts specifically with human Substance P. No crossreactivity with related tachykinins (human Neurokinin A, Neurokinin B, and Kassinin).
• Tested applicationsELISA, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Rat, Guinea pig, Human
  Predicted to work with: Mouse, Horse, Chicken, Hamster, Cow, Shark
• Immunogen

  Synthetic peptide: RPRPQQFFGLM, corresponding to amino acids 1-11 of Human Substance P.

  Run BLAST with Run BLAST with
• Positive controlGuinea pig bone marrow. The antibody binds to the substantia gelatinosa.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS, 0.1M Glycine. pH 7.4
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone numberSP-DE4-21
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Neuroscience
  • Sensory System
  • Somatosensory system
  • Nociception

  • Neuroscience
  • Neurotransmitter
  • Neuropeptides
  • Tachykinins

  • Neuroscience
  • Neurology process
  • Metabolism

  • Neuroscience
  • Neurotransmitter
  • Neuropeptides
  • Hormones

Applications

Target

• FunctionTachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
• Sequence similaritiesBelongs to the tachykinin family.
• Cellular localizationSecreted.

Target information above from: UniProt accession P20366 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6863 Human
  • Entrez Gene: 21333 Mouse
  • Entrez Gene: 24806 Rat
  • Omim: 162320 Human
  • SwissProt: P20366 Human
  • SwissProt: P41539 Mouse
  • SwissProt: P06767 Rat
  • Unigene: 2563 Human

  • Unigene: 1440 Mouse
  • Unigene: 410823 Mouse
  • Unigene: 1920 Rat

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• Alternative names
  C-terminal-flanking peptide antibodyHs.2563 antibodyNeurokinin 1 antibody

  Neurokinin 2 antibodyNeurokinin A antibodyNeurokinin alpha antibodyNeuromedin L antibodyNeuropeptide gamma antibodyNeuropeptide K antibodyNK2 antibodyNKA antibodyNKNA antibodyNPK antibodyPPT antibodyProtachykinin 1 precursor antibodySubstance K antibodySubstanceP antibodyTAC1 antibodyTAC2 antibodyTachykinin 1 antibodyTachykinin 2 antibodyTachykinin precursor 1 antibodyTachykinin1 antibodyTACI antibodyTKN1_HUMAN antibody

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Anti-Substance P antibody [SP-DE4-21] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)
  Substance P staining at 1:1000 of the substancia gelatinosa (dorsal horn) of the guinea pig spinal cord using an HRP-conjugated secondary antibody and DAB.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (ab14184)
  ICC/IF image of ab14184 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14184, 1ug/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)Image courtesy of an anonymous Abreview.
  ab14184 staining Substance P in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  
  Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed. Samples were then incubated with ab14184 at a 1/100 dilution for 1 hour. The secondary used was an undiluted HRP conjugated goat anti-mouse polyclonal.

  See Abreview

• 

  ELISA - Anti-Substance P antibody [SP-DE4-21] (ab14184)This image is courtesy of an anonymous Abreview
  ab14184 detecting Substance P in Human Serum samples by sandwich ELISA. Samples were blocked with 1% BSA for 1 hour at 25°C and incubated with the primary antibody (1/400 in PBS) for 20 hours at 4°C. A biotinylated Rabbit anti-Substance P polyclonal (1/200) was used as the secondary antibody.

  See Abreview

Protocols

• Recommended protocol for IHC-P with ab14184