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Product name
Succinate Dehydrogenase (Complex II) Human Profiling ELISA Kit
See all Complex II products (5) ...
Specificity
Species – Human. Rat and Mouse samples are not appropriate, other species are untested.
Precision
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Overall | 5 | 3.9% |
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Overall | 3 | 15% |
Tests
1 x 96 test
Sample type
Tissue Extracts, Cell Lysate
Assay type
Sandwich
Sensitivity
50 µg/ml
Recovery
| Sample type | Average % | Range % |
|---|---|---|
| Tissue Extracts | 12 - 200 | |
| Cell Lysate | 50 - 1000 |
Cross reactivity
Reacts with
Human
Product overview
Succinate Dehydrogenase (Complex II) is the second enzyme of the oxidative phosphorylation (OXPHOS) system within the mitochondrial inner membrane. Succinate dehydrogenase is a large protein complex of 140,000 MW made up of 4 different subunits (SDH, SDB, SDHC, and SDHD). The enzyme forms a dimer in the mitochondrial inner membrane. Succinate Dehydrogenase is the only enzyme of the OXPHOS system containing no mitochondrial DNA (mtDNA) encoded subunit components. Therefore all four subunits are encoded by nuclear genomic DNA, made in the cytosol, and translocated into the organelle for assembly at the inner membrane. The enzyme complex catalyses electron entry from succinate via a flavin adenine dinucleotide (FAD), three iron sulfur clusters and b-type cytochrome. Succinate Dehydrogenase also participates in the citric acid cycle producing fumarate for the next step in the cycle - hydration by fumarase.
ab124536 Succinate Dehydrogenase (SDH, Complex II) Human Profiling ELISA Kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the comparison of succinate dehydrogenase levels or profile in cell and tissue lysates. The assay employs a the human succinate dehydrogenase complex specific coated onto microplate well plate strips.
Samples are pipetted into the wells and succinate dehydrogenase present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-succinate dehydrogenase detector antibody is added. After washing away unbound detector antibody, an HRP-conjugated secondary antibody specific for the detector antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of succinate dehydrogenase (Complex II) bound. The developing blue color is measured at 600 nm.
Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Notes
Store all components at 4°C. This kit is stable for 6 months from receipt. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
Tested applications
Sandwich ELISAmore details
Properties
Storage instructions
Please see Notes section
| Components | Identifier | 1 x 96 tests |
|---|---|---|
| 10X Blocking Buffer | 8209802 | 1 x 6ml |
| 10X HRP Label | 8209562 | 1 x 1ml |
| 10X SDH Detector Antibody | 8209561 | 1 x 0.75ml |
| 20X Buffer | 8203013 | 1 x 20ml |
| Extraction Buffer | 8201093 | 1 x 15ml |
| SDH Microplate | 8209563 | 1 unit |
| TMB Development Solution | 8209803 | 1 x 12ml |
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Sandwich ELISA - Succinate Dehydrogenase (Complex II) Human Profiling ELISA Kit (ab124536)
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Sandwich ELISA - Succinate Dehydrogenase (Complex II) Human Profiling ELISA Kit (ab124536)
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Sandwich ELISA - Succinate Dehydrogenase (Complex II) Human Profiling ELISA Kit (ab124536)
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Relevance
Complex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.
Alternative names
- MS1036
- MS241
- MTOX2
- Succinate coenzyme Q reductase
Database links
- Entrez Gene: 6390 Human
- Entrez Gene: 6389 Human
- Entrez Gene: 6391 Human
- SwissProt: P31040 Human
- SwissProt: P21912 Human
- SwissProt: Q99643 Human
- SwissProt: O14521 Human
- SwissProt: 6392 Human
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab124536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA
Succinate Dehydrogenase (Complex II) Human Profiling ELISA Kit images:
Sandwich ELISA - Succinate Dehydrogenase (Complex II) Human Profiling ELISA Kit (ab124536)
Example control sample curve of serially titrated HepG2 lysate in the working range of the assay.
Sandwich ELISA - Succinate Dehydrogenase (Complex II) Human Profiling ELISA Kit (ab124536)
Example control sample curve of serially titrated human heart tissue homogenate in the working range of the assay.
Sandwich ELISA - Succinate Dehydrogenase (Complex II) Human Profiling ELISA Kit (ab124536)
Example control sample curve of serially titrated HL60 lysate in the working range of the assay.
Sandwich ELISA - Succinate Dehydrogenase (Complex II) Human Profiling ELISA Kit (ab124536)
Human HepG2 cells were cultured in NARTI Zalcitabine (ddC) for 6 days to ensure a significant effect on mitochondrial DNA replication and mitochondrial protein translation, respectively. The drug reduced mitochondrial DNA levels and hence mitochondrial protein expression. As a consequence the assembly of Complexes I, III and IV were severely affected. Note that loss of the two small mitochondrial DNA encoded subunits of Complex V (ATP synthase) does not affect overall assembly. Interestingly an increase in Complex II was induced as a consequence of I, III, IV loss possibly to up regulate mitochondrial citric acid cycle function.
Sandwich ELISA - Succinate Dehydrogenase (Complex II) Human Profiling ELISA Kit (ab124536)
Human HepG2 cells were cultured in chloramphenicol for 6 days to ensure a significant effect on mitochondrial DNA replication and mitochondrial protein translation, respectively. The antibiotic inhibited mitochondrial protein translation and assembly of Complexes I and IV but had no significant effect on Complex II, III or V.
Protocols
References for Succinate Dehydrogenase (Complex II) Human Profiling ELISA Kit (ab124536)
ab124536 has not yet been referenced specifically in any publications.
Publishing research using ab124536? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"