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- Proteins and Peptides
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Product name
Anti-Sumo 1 antibody
See all Sumo 1 products (13) ...
Description
Sheep polyclonal to Sumo 1
Specificity
Reacts with Human SUMO-1, no cross-reactivity with ubiquitin.
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Synthetic peptide: AKPSTEDLGDKKEGEY, corresponding to amino acids 6-21 of Human Sumo 1 conjugated to Diphtheria toxoid via a cysteine residue added at the Carboxyl terminus of the peptide.
AKPSTEDLGDKKEGEY
Epitope
The sequence used was AKPSTEDLGDKKEGEY.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Autophagy and mitophagy >> APG gene products
Cardiovascular >> Heart >> Autophagy >> APG gene products
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Sumo
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/8000. Predicted molecular weight: 12 kDa.
IHC-P
IHC-P: 1/4000.IHC-P: 1/4000.
Target
Function
Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.
Involvement in disease
Defects in SUMO1 are the cause of non-syndromic orofacial cleft type 10 (OFC10) [MIM:613705]; also called non-syndromic cleft lip with or without cleft palate 10. OFC10 is a birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. Note=A chromosomal aberation involving SUMO1 is the cause of OFC10. Translocation t(2;8)(q33.1;q24.3). The breakpoint occurred in the SUMO1 gene and resulted in haploinsufficiency confirmed by protein assays.
Sequence similarities
Belongs to the ubiquitin family. SUMO subfamily.
Contains 1 ubiquitin-like domain.
Post-translational
modifications
Cleavage of precursor form by SENP1 or SENP2 is necessary for function.
Polymeric SUMO1 chains undergo polyubiquitination by RNF4.
Cellular localization
Nucleus membrane. Nucleus speckle. Cytoplasm. Recruited by BCL11A into the nuclear body.
Target information above from: UniProt accessionP63165
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- GAP modifying protein 1 antibody
- GAP-modifying protein 1 antibody
- GMP 1 antibody
see all
Database links
- Entrez Gene: 7341 Human
- Entrez Gene: 301442 Rat
- Omim: 601912 Human
- SwissProt: P63165 Human
- SwissProt: Q5I0H3 Rat
- Unigene: 596171 Human
- Unigene: 81424 Human
- Unigene: 1221 Rat
References for Anti-Sumo 1 antibody (ab22738)
ab22738 has not yet been referenced specifically in any publications.
Publishing research using ab22738? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"