Anti-Sumo 1 antibody (ab5316)
Key features and details
- Rabbit polyclonal to Sumo 1
- Suitable for: WB
- Reacts with: Arabidopsis thaliana
- Isotype: IgG
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Overview
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Product name
Anti-Sumo 1 antibody
See all Sumo 1 primary antibodies -
Description
Rabbit polyclonal to Sumo 1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Arabidopsis thaliana -
Immunogen
Recombinant full length protein corresponding to Arabidopsis thaliana Sumo 1 aa 1 to the C-terminus.
Database link: P55852 -
Positive control
- Arabidopsis extracts that have been heat treated for for 30-120 mins by moving them from 24 to 37 degrees C. Recombinant SUMO1 is detectable at concentrations as low as 5ng/ml.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000. Predicted molecular weight: 12 kDa.
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Notes |
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WB
1/1000. Predicted molecular weight: 12 kDa. |
Target
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Function
Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. -
Involvement in disease
Defects in SUMO1 are the cause of non-syndromic orofacial cleft type 10 (OFC10) [MIM:613705]; also called non-syndromic cleft lip with or without cleft palate 10. OFC10 is a birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. Note=A chromosomal aberation involving SUMO1 is the cause of OFC10. Translocation t(2;8)(q33.1;q24.3). The breakpoint occurred in the SUMO1 gene and resulted in haploinsufficiency confirmed by protein assays. -
Sequence similarities
Belongs to the ubiquitin family. SUMO subfamily.
Contains 1 ubiquitin-like domain. -
Post-translational
modificationsCleavage of precursor form by SENP1 or SENP2 is necessary for function.
Polymeric SUMO1 chains undergo polyubiquitination by RNF4. -
Cellular localization
Nucleus membrane. Nucleus speckle. Cytoplasm. Recruited by BCL11A into the nuclear body. - Information by UniProt
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Alternative names
- DAP1 antibody
- GAP modifying protein 1 antibody
- GAP-modifying protein 1 antibody
see all
Datasheets and documents
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Datasheet download
References (36)
ab5316 has been referenced in 36 publications.
- Li X et al. Fine-tuning of SUMOylation modulates drought tolerance of apple. Plant Biotechnol J 20:903-919 (2022). PubMed: 34978131
- Han D et al. Chromatin-associated SUMOylation controls the transcriptional switch between plant development and heat stress responses. Plant Commun 2:100091 (2021). PubMed: 33511343
- Watson SJ et al. Crosstalk between the chloroplast protein import and SUMO systems revealed through genetic and molecular investigation in Arabidopsis. Elife 10:N/A (2021). PubMed: 34473053
- Dong Y et al. HEARTBREAK Controls Post-translational Modification of INDEHISCENT to Regulate Fruit Morphology in Capsella. Curr Biol 30:3880-3888.e5 (2020). PubMed: 32795439
- Qu GP et al. Reversible SUMOylation of FHY1 Regulates Phytochrome A Signaling in Arabidopsis. Mol Plant 13:879-893 (2020). PubMed: 32298785