Overview
- Product nameAnti-Sumo 1 antibodySee all Sumo 1 primary antibodies ...
- DescriptionRabbit polyclonal to Sumo 1
- Tested applicationsWB more details
- Species reactivityReacts with: Arabidopsis thaliana
- Immunogen
Recombinant full length protein (Arabidopsis thaliana).
- Positive controlArabidopsis extracts that have been heat treated for for 30-120 mins by moving them from 24 to 37 degrees C. Recombinant SUMO1 is detectable at concentrations as low as 5ng/ml.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- PurityWhole antiserum
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab5316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/1000. Predicted molecular weight: 12 kDa. |
Target
- FunctionUbiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.
- Involvement in diseaseDefects in SUMO1 are the cause of non-syndromic orofacial cleft type 10 (OFC10) [MIM:613705]; also called non-syndromic cleft lip with or without cleft palate 10. OFC10 is a birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. Note=A chromosomal aberation involving SUMO1 is the cause of OFC10. Translocation t(2;8)(q33.1;q24.3). The breakpoint occurred in the SUMO1 gene and resulted in haploinsufficiency confirmed by protein assays.
- Sequence similaritiesBelongs to the ubiquitin family. SUMO subfamily.
Contains 1 ubiquitin-like domain. - Post-translational
modificationsCleavage of precursor form by SENP1 or SENP2 is necessary for function.
Polymeric SUMO1 chains undergo polyubiquitination by RNF4. - Cellular localizationNucleus membrane. Nucleus speckle. Cytoplasm. Recruited by BCL11A into the nuclear body.
Target information above from: UniProt accession
P63165
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- GAP modifying protein 1 antibodyGAP-modifying protein 1 antibodyGMP 1 antibody
- GMP1 antibodyOFC10 antibodyPIC 1 antibodyPIC1 antibodySENP2 antibodySentrin 1 antibodySentrin antibodySmall ubiquitin related modifier 1 antibodySmall ubiquitin-related modifier 1 antibodySMT3 antibodySMT3 antibodySMT3 homolog 3 antibodySMT3 suppressor of mif two 3 homolog 1 antibodySMT3C antibodySMT3H3 antibodySUMO-1 antibodySumo1 antibodySUMO1_HUMAN antibodyUbiquitin homology domain protein PIC1 antibodyUbiquitin Like 1 antibodyUbiquitin like protein SMT3C antibodyUbiquitin like protein UBL1 antibodyUbiquitin-homology domain protein PIC1 antibodyUbiquitin-like protein SMT3C antibodyUbiquitin-like protein UBL1 antibodyUBL 1 antibodyUBL1 antibody
see all
Anti-Sumo 1 antibody images
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Western blotWestern blots of SUMO1 and SUMO3 antibody on arabidopsis extracts heat treated for the indicated times by moving them from 24 to 37 degrees C. The panel on the left was using ab5316, the panel on the right was using ab5317 - both antibodies were used at a dilution of 1/1000.
References for Anti-Sumo 1 antibody (ab5316)
This product has been referenced in:
- Hermkes R et al. Distinct roles for Arabidopsis SUMO protease ESD4 and its closest homolog ELS1. Planta 233:63-73 (2011). WB ; Arabidopsis thaliana . Read more (PubMed: 20922545) »
- Budhiraja R et al. Substrates related to chromatin and to RNA-dependent processes are modified by Arabidopsis SUMO isoforms that differ in a conserved residue with influence on desumoylation. Plant Physiol 149:1529-40 (2009). WB ; Arabidopsis thaliana . Read more (PubMed: 19151129) »
