![Western blot - Sumo 1 antibody [none] (ab93951) image](#Sumo-1-Primary-antibodies-ab93951-1.jpg)
![Immunocytochemistry/ Immunofluorescence - Sumo 1 antibody [none] (ab93951) image](#Sumo-1-Primary-antibodies-ab93951-2.jpg)
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Product name
Anti-Sumo 1 antibody [none]
See all Sumo 1 products (13) ...
Description
Mouse monoclonal [none] to Sumo 1
Tested applications
WB, IP, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length mature Sumo 1 protein (Human) with N terminal 6x His Tag
Positive control
HeLa cells or cell lysate
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Monoclonal
Clone number
none
Isotype
IgG1
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Autophagy and mitophagy >> APG gene products
Cardiovascular >> Heart >> Autophagy >> APG gene products
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Sumo
Applications
Show applications key
Our Abpromise guarantee covers the use of ab93951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 12 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.
Involvement in disease
Defects in SUMO1 are the cause of non-syndromic orofacial cleft type 10 (OFC10) [MIM:613705]; also called non-syndromic cleft lip with or without cleft palate 10. OFC10 is a birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. Note=A chromosomal aberation involving SUMO1 is the cause of OFC10. Translocation t(2;8)(q33.1;q24.3). The breakpoint occurred in the SUMO1 gene and resulted in haploinsufficiency confirmed by protein assays.
Sequence similarities
Belongs to the ubiquitin family. SUMO subfamily.
Contains 1 ubiquitin-like domain.
Post-translational
modifications
Cleavage of precursor form by SENP1 or SENP2 is necessary for function.
Polymeric SUMO1 chains undergo polyubiquitination by RNF4.
Cellular localization
Nucleus membrane. Nucleus speckle. Cytoplasm. Recruited by BCL11A into the nuclear body.
Target information above from: UniProt accessionP63165
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- GAP modifying protein 1 antibody
- GAP-modifying protein 1 antibody
- GMP 1 antibody
see all
Database links
- Entrez Gene: 7341 Human
- Omim: 601912 Human
- SwissProt: P63165 Human
- Unigene: 596171 Human
- Unigene: 81424 Human
Anti-Sumo 1 antibody [none] images:
Western blot - Sumo 1 antibody [none] (ab93951)
![Western blot - Sumo 1 antibody [none] (ab93951)](/ps/datasheet/images/93/ab93951/Sumo-1-Primary-antibodies-ab93951-1.jpg)
Predicted band size : 12 kDa
Hela cells were arrested in S phase using thymidine, or synchronized in mitosis using thymidine and nocodazole block followed by release from nocodazole for the indicated times (hours). Cell lysates were separated by SDS-PAGE and analyzed with ab93951.
Immunocytochemistry/ Immunofluorescence - Sumo 1 antibody [none] (ab93951)
![Immunocytochemistry/ Immunofluorescence - Sumo 1 antibody [none] (ab93951)](/ps/datasheet/images/93/ab93951/Sumo-1-Primary-antibodies-ab93951-2.jpg)
HeLa cells were permeabilized with digitonin, fixed with formaldheyde and analyzed by IF confocal microscopy using ab93951. Indicated cell cycle stages were defined by DNA staining with DAPI. Bar equals 5µm.
References for Anti-Sumo 1 antibody [none] (ab93951)
This product has been referenced in:
- Zhang XD et al. SUMO-2/3 modification and binding regulate the association of CENP-E with kinetochores and progression through mitosis. Mol Cell 29:729-41 (2008). WB, ICC/IF.Read more (PubMed: 18374647) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"