![Western blot - Sumo 2+3 antibody [8A2] (ab81371) image](#Sumo-23-Primary-antibodies-ab81371-1.jpg)
![Immunocytochemistry/ Immunofluorescence - Sumo 2+3 antibody [8A2] (ab81371) image](#Sumo-23-Primary-antibodies-ab81371-2.jpg)
 image](#Sumo-23-Primary-antibodies-ab81371-3.jpg)
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Product name
Anti-Sumo 2+3 antibody [8A2]
See all Sumo 2+3 products (6) ...
Description
Mouse monoclonal [8A2] to Sumo 2+3
Specificity
This antibody recognizes Sumo 2 and 3 but does not recognize Sumo 1.
Tested applications
WB, ICC/IF, IP, ChIP, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length Sumo 2 protein (Human)
Positive control
HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
8A2
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Sumo
Applications
Show applications key
Our Abpromise guarantee covers the use of ab81371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000. Predicted molecular weight: 12 kDa.
ICC/IF: 1/2000.
IP: Use at an assay dependent dilution.
ChIP: Use at an assay dependent concentration.
IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Relevance
SUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.
Cellular localization
Cytoplasmic (SUMO3) and Nuclear (SUMO2)
Alternative names
- HSMT3 antibody
- MGC117191 antibody
- OTTHUMP00000115275 antibody
see all
Database links
- Entrez Gene: 6613 Human
- Entrez Gene: 6612 Human
- Omim: 603042 Human
- Omim: 602231 Human
- SwissProt: P61956 Human
- SwissProt: P55854 Human
Anti-Sumo 2+3 antibody [8A2] images:
Western blot - Sumo 2+3 antibody [8A2] (ab81371)
![Western blot - Sumo 2+3 antibody [8A2] (ab81371)](/ps/datasheet/images/81/ab81371/Sumo-23-Primary-antibodies-ab81371-1.jpg)
Anti-Sumo 2+3 antibody [8A2] (ab81371) at 1/2000 dilution + HeLa cell lysate
Predicted band size : 12 kDa
Observed band size : 12 kDa
Immunocytochemistry/ Immunofluorescence - Sumo 2+3 antibody [8A2] (ab81371)
![Immunocytochemistry/ Immunofluorescence - Sumo 2+3 antibody [8A2] (ab81371)](/ps/datasheet/images/81/ab81371/Sumo-23-Primary-antibodies-ab81371-2.jpg)
Ab81371 (1:2000) staining human Sumo 2 + 3 in HeLa cells by immunofluorescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Sumo 2+3 antibody [8A2](ab81371)
](/ps/datasheet/images/81/ab81371/Sumo-23-Primary-antibodies-ab81371-3.jpg)
IHC image of ab81371 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab81371, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Sumo 2+3 antibody [8A2] (ab81371)
This product has been referenced in:
- Ismail IH et al. CBX4-mediated SUMO modification regulates BMI1 recruitment at sites of DNA damage. Nucleic Acids Res : (2012). ICC/IF; Human.Read more (PubMed: 22402492) »
- Liang Q et al. Tripartite motif-containing protein 28 is a small ubiquitin-related modifier E3 ligase and negative regulator of IFN regulatory factor 7. J Immunol 187:4754-63 (2011). WB.Read more (PubMed: 21940674) »
See all 4 publications for this product
Publishing research using ab81371? Please let us know so that we can cite the reference in this datasheet
![Anti-Sumo 2+3 antibody [8A2] for ChIP in Human (81371)](/ps/datasheet/images/81/ab81371/Sumo-23-Primary-antibodies-ab81371-6.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"