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Product name
Anti-Sumo 2+3 antibody
See all Sumo 2+3 products (6) ...
Description
Rabbit polyclonal to Sumo 2+3
Specificity
Recognises 2 bands representing Sumo 2 and Sumo 3 at 15 and 18kDa in Hela Nuclear extract by Western blotting.
Tested applications
ICC/IF, WB, IF, IHC-P, ICCmore details
Cross reactivity
Reacts with
Mouse, Human, Chinese Hamster
Predicted to work with
Rat, Cow, Pig, Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Sumo 2+3.
(Peptide available as ab137 60.)
Positive control
This antibody gave a positive signal in HeLa Nuclear cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Sumo
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration.
WB: 1/1000. Detects a band of approximately 15 , 18 kDa (predicted molecular weight: 11.6 , 10.8 kDa).Can be blocked with Sumo 2+3 peptide (ab13760).
IF: 1/100.
IHC-P: 1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC
ICC: 1/100.ICC: 1/100.
Target
Relevance
SUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.
Cellular localization
Cytoplasmic (SUMO3) and Nuclear (SUMO2)
Alternative names
- HSMT3 antibody
- MGC117191 antibody
- OTTHUMP00000115275 antibody
see all
Database links
- Entrez Gene: 6613 Human
- Entrez Gene: 6612 Human
- Entrez Gene: 20610 Mouse
- Entrez Gene: 170930 Mouse
- Entrez Gene: 499417 Rat
- Entrez Gene: 690244 Rat
- Omim: 603042 Human
- Omim: 602231 Human
see all
Anti-Sumo 2+3 antibody images:
Western blot
Lanes 1 & 3 : Anti-Sumo 2+3 antibody (ab3742) at 1/500 dilution Predicted band sizes : 11.6 & 10.8 kDa Detects 2 bands representing Sumo 2 and Sumo 3.
Lanes 2 & 4 : Anti-Sumo 2+3 antibody (ab3742) at 1/1000 dilution
Lane 1 : HeLa nuclear extract
Lane 2 : HeLa nuclear extract
Lane 3 : HeLa nuclear extract with
Lane 4 : HeLa nuclear extract with
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 15,18 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
Observed band sizes : 15 & 18 kDa
Immunofluorescence - Sumo 2+3 antibody (ab3742)
Immunofluorescent imaging of human cells (U2OS) with ab3742 confirms the specificity of this antibody. Antibody signal is localised exclusively to the nucleus, with diffuse background staining of the nucleoplasm. Intense foci of staining are also evident, corresponding to SUMO-2/3 accumulation in nuclear subdomains such as the PML body. This image is in exact agreement with several published reports (see for example Saitoh H et al.).
IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees. Nuclei are stained with Hoechst stain.
Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK
- Sumo 2+3 antibody (ab3742)
Image courtesy of Human Protein Atlas
ab3742 staining Sumo 2 + 3 in Human skin. The paraffin embedded human skin tissue was incubated with ab3742 (1/800 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab3742 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
References for Anti-Sumo 2+3 antibody (ab3742)
This product has been referenced in:
- Lukas C et al. 53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of chromosomes under replication stress. Nat Cell Biol 13:243-53 (2011). ICC/IF; Human.Read more (PubMed: 21317883) »
- Plant LD et al. SUMO modification of cell surface Kv2.1 potassium channels regulates the activity of rat hippocampal neurons. J Gen Physiol 137:441-54 (2011). Rat.Read more (PubMed: 21518833) »
See all 12 publications for this product
Publishing research using ab3742? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"