Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
- Lysates
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-Sumo 3 antibody
See all Sumo 3 products (8) ...
Description
Rabbit polyclonal to Sumo 3
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 54-103 (MRQIRFRFDG QPINETDTPA QLEMEDEDTI DVFQQQTGGV PESSLAGHSF) of Human Sumo 3 (NP_008867).
MRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGGV PESSLAGHSF
Positive control
MCF7 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Sumo
-
Western blot - Sumo 3 antibody (ab85442)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab85442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 12 kDa.Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Target
Function
Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4.
Tissue specificity
Expressed predominantly in liver.
Sequence similarities
Belongs to the ubiquitin family. SUMO subfamily.
Contains 1 ubiquitin-like domain.
Post-translational
modifications
Polymeric chains can be formed through Lys-11 cross-linking.
Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP55854
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Small ubiquitin related modifier 3 antibody
- Small ubiquitin-related modifier 3 antibody
- SMT3 homolog 1 antibody
see all
Database links
- Entrez Gene: 6612 Human
- Omim: 602231 Human
- SwissProt: P55854 Human
- Unigene: 474005 Human
Anti-Sumo 3 antibody images:
Western blot - Sumo 3 antibody (ab85442)
Anti-Sumo 3 antibody (ab85442) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 12 kDa
Observed band size : 12 kDa
Gel concentration: 10-20%
References for Anti-Sumo 3 antibody (ab85442)
ab85442 has not yet been referenced specifically in any publications.
Publishing research using ab85442? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"