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Full length protein (Human).
Our Abpromise guarantee covers the use of ab13498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000. See Abreview.|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||Use a concentration of 0.2 µg/ml. Detects a band of approximately 19, 23 kDa (predicted molecular weight: 18 kDa). See Kurobe et al, 1990, Clinica Chimica Acta 187: 11-20 and Shinder et al 2001.|
|IP||Use a concentration of 10 µg/ml. See Shinder et al 2001.|
|ELISA||Use at an assay dependent concentration. See Downs et al 2002.|
|IHC-P||Use at an assay dependent concentration.|
ab13498 at 1µg/ml staining Superoxide dismutase 1 in human placenta tissue section by Immunohistochemistry ((Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A HRP conjugated goat anti-rabbit secondary was used at 1/10 dilution.
This image is courtesy of an Abreview submitted by Gwen Lagoda
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