Anti-Superoxide Dismutase 1 antibody (ab13498)

Overview

  • Product nameAnti-Superoxide Dismutase 1 antibodySee all Superoxide Dismutase 1 primary antibodies ...
  • Description
    Rabbit polyclonal to Superoxide Dismutase 1
  • Tested applicationsICC/IF, IHC-Fr, WB, IP, ELISA, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Fruit fly (Drosophila melanogaster), Monkey, African Green Monkey
  • Immunogen

    Full length protein (Human).

  • Positive control
    • HeLa Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF 1/1000. See Abreview.
IHC-Fr 1/1000.
WB Use a concentration of 0.2 µg/ml. Detects a band of approximately 19, 23 kDa (predicted molecular weight: 18 kDa). See Kurobe et al, 1990, Clinica Chimica Acta 187: 11-20 and Shinder et al 2001.
IP Use a concentration of 10 µg/ml. See Shinder et al 2001.
ELISA Use at an assay dependent dilution. See Downs et al 2002.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionDestroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • Involvement in diseaseDefects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
  • Sequence similaritiesBelongs to the Cu-Zn superoxide dismutase family.
  • Post-translational
    modifications
    Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
    The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
  • Cellular localizationCytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
  • Target information above from: UniProt accession P00441 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
    • ALS 1 antibody
    • ALS antibody
    • ALS1 antibody
    • Amyotrophic lateral sclerosis 1 adult antibody
    • Amyotrophic lateral sclerosis 1 antibody
    • Cu/Zn SOD antibody
    • Cu/Zn superoxide dismutase antibody
    • Homodimer antibody
    • hSod1 antibody
    • Indophenoloxidase A antibody
    • IPOA antibody
    • Mn superoxide dismutase antibody
    • SOD 1 antibody
    • SOD antibody
    • SOD soluble antibody
    • SOD1 antibody
    • SOD2 antibody
    • SODC antibody
    • SODC_HUMAN antibody
    • Soluble indophenoloxidase A antibody
    • Superoxide dismutase [Cu-Zn] antibody
    • Superoxide dismutase 1 antibody
    • Superoxide dismutase 1 soluble antibody
    • Superoxide dismutase Cu Zn antibody
    • Superoxide dismutase cystolic antibody
    see all

Anti-Superoxide Dismutase 1 antibody images

  • ab13498 (1/200) staining Human cell line 293FT by ICC/IF. The 293 FT cells were cultured for 3 days, fixed in 3.7% formaldehyde and blocked with 5% BSA in PBS for 1 hr. The secondary antibody was goat anti-Rabbit IgG conjugated to Alexa Fluor® 488 (green) and the nucleus (blue) were stained with DAPI.
  • ab13498 at 1µg/ml staining Superoxide dismutase 1 in human placenta tissue section by Immunohistochemistry ((Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A HRP conjugated goat anti-rabbit secondary was used at 1/10 dilution.

  • ab13498 staining Superoxide Dismutase 1 in rat brain tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with formaldehyde and then blocked with 2% BSA for 2 hours at 25°C followed by incubation with the primary antibody, at a 1/1000 dilution, for 9 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.

    See Abreview

  • ab5103 staining Histone H3 (citrulline 2 + 8 + 17) in Mouse bone marrow white blood cells by Immunocytochemistry/ Immunoflourescence. Cells were fixed in formaldehyde and permeabilized in 0.1% Triton X-100 prior to blocking in 5% Goat serum for 2 hours at 25°C. The primary antibody was diluted 1/500 in PBS and incubated with the sample for 12 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/500.
    Nuclei were counterstained blue with DAPI.

    See Abreview

  • ICC/IF image of ab13498 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13498, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Superoxide Dismutase 1 antibody (ab13498) at 1/1000 dilution (in starting block T20 (PBS) for 18 hours at 4°C )

    Lane 1 : Whole tissue lysate of human penile tissue
    Lane 2 : Whole tissue lysate of human penile tissue
    Lane 3 : Whole tissue lysate of human penile tissue
    Lane 4 : Whole tissue lysate of human penile tissue

    Lysates/proteins at 60 µg per lane.

    Secondary
    An HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution

    Predicted band size : 18 kDa
    Observed band size : 18 kDa

    This image is courtesy of an Abreview submitted by Gwen Lagoda

References for Anti-Superoxide Dismutase 1 antibody (ab13498)

This product has been referenced in:
  • Tang JR  et al. The NF-?B inhibitory proteins I?Ba and I?Bß mediate disparate responses to inflammation in fetal pulmonary endothelial cells. J Immunol 190:2913-23 (2013). Rat . Read more (PubMed: 23418625) »
  • Yue F  et al. Maternal transfer of immunity in scallop Chlamys farreri and its trans-generational immune protection to offspring against bacterial challenge. Dev Comp Immunol 41:569-77 (2013). WB . Read more (PubMed: 23856157) »

See all 11 Publications for this product

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ab13498 - ***
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Expiration date: March 12, 2013

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Please check the online datasheets of all antibodies. You will find the required information.

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The possible rabbit specific antibodies are available as;


ICAM1: the rabbit protein sequence (http://www.uniprot.org/uniprot/Q75T02) does not show any homology with human protein (http:...

Read More
Application Western blot
Sample Rat Tissue lysate - whole (hypocamp foetal)
Loading amount 20 µg
Specification hypocamp foetal
Gel Running Conditions Reduced Denaturing (15)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 29 2012

Thank you for contacting Abcam.

We currently have 418 products that have cross reactivity records for Saccharomyces cerevisiaeon our catalog. While we try to test all of our antibodies in a wide range of species we cannot test each in every ...

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Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Loading amount 30 µg
Specification Skeletal muscle
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 32°C
Username

Mr. Mtuhtu S.Gounder

Verified customer

Submitted Jan 06 2012

Application Western blot
Sample Human Tissue lysate - whole (Human Penile Tissue)
Loading amount 60 µg
Specification Human Penile Tissue
Gel Running Conditions Reduced Denaturing (4-20% mini gel)
Blocking step Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. gwen Lagoda

Verified customer

Submitted Oct 26 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Bone marrow WBCs)
Specification Bone marrow WBCs
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 16 2011

Application Western blot
Sample Mouse Tissue lysate - whole (Mouse Penile Tissue)
Loading amount 30 µg
Specification Mouse Penile Tissue
Gel Running Conditions Reduced Denaturing (5% mini gel)
Blocking step Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. gwen Lagoda

Verified customer

Submitted Jan 25 2011

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"