Superoxide Dismutase 1 protein (Human) (ab82649)
Constituents: 20mM HEPES, pH 7.4
Our Abpromise guarantee covers the use of ab82649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|SDS-PAGE||SDS-PAGE: Use at an assay dependent dilution.|
|WB||WB: Use at an assay dependent concentration. Ab82649 can be used as a WB positive control in conjunction with ab88404.|
- ALSALS1Amyotrophic lateral sclerosis 1 adult
- Cu/Zn SODCu/Zn superoxide dismutaseHomodimerhSod1Indophenoloxidase AIPOAMn superoxide dismutaseSODSOD solubleSOD1SOD2SODCSODC_HUMANSuperoxide dismutase [Cu-Zn]Superoxide dismutase 1Superoxide dismutase 1 solubleSuperoxide dismutase Cu ZnSuperoxide dismutase cystolic
modificationsUnlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
Superoxide Dismutase 1 protein (Human) images
ab82649 (1µg) on SDS-PAGE.
All lanes : Anti-Superoxide Dismutase 1 antibody (ab88404) at 1 µg/ml
Lane 1 :
Superoxide Dismutase 1 protein (Human) (ab82649) at 0.01 µg
Lane 2 :
Superoxide Dismutase 1 protein (Human) (ab82649) at 0.001 µg
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 30 seconds
References for Superoxide Dismutase 1 protein (Human) (ab82649)
ab82649 has not yet been referenced specifically in any publications.