You have changed your country from to
. Please be aware that this will change the currency in the purchasing process.
- Primary antibodies
- Secondary antibodies
-
Proteins & Peptides
-
A-Z
- Full length proteins
- Partial proteins
- Peptides
-
Enzymes
- MMPs
- Caspases
- Kinases and transferases
- Signal transduction related
-
Cytokines and growth factors
- Cytokines
- Growth factors by family
-
Human proteins
- Human expressed
- Barley expressed
- Wheat expressed
- Fc fusion proteins
- Ig proteins
-
His-tagged Proteins by research area
- Cancer
- Cardiovascular
- Epigenetics & Nuclear Signaling
- Cell Biology
- Immunology
- Neuroscience
- Signal Transduction
- Stem Cells
-
Microbiology and virology
- All microbiology and viral proteins
- DNA virus proteins
- RNA viral proteins
- All Hepatitis virus proteins
- HIV proteins
- HSV, Measles and Influenza proteins
- Bacterial toxins and chaperones
-
Resources
- Advantages of expression in barley
-
-
ELISA Kits
-
A-Z
- ELISA Kits
- ELIPAIR Kits
- ELISPOT Kits
- FLUOROSPOT Kits
-
ELISA development
- Matched antibody pairs
-
Resources
- Immunoassay technical know-how
- ELISA and ELIPAIR
- ELISPOT and FLUOROSPOT
-
ELISA Kits by protein family
- Adaptive immune components
- Adhesion molecules
- Angiogenic factors
- Apoptosis markers
- Atherosclerotic proteins
- Blood cell antigens
- Cancer metabolism proteins
- Cardiovascular proteins
- CD markers
- Cell cycle proteins
- Complement activation
- Cytokines and cytokine receptors
- Developmental proteins
- Granzymes and perforins
- Growth factors/hormones
- Immunoglobulins
- Oncoproteins
- Pathogen specific immunoglobulins
- Protease inhibitors
- Signal transduction proteins
- Sub cellular markers
- Tumor biomarkers
-
-
Assay Kits
-
A-Z
- Apoptosis kits
- Epigenetic kits
- Metabolism kits
- ChIP kits
-
Metabolism kits by analyte
- Alcohol
- Amino acids
- Calcium
- Cell viability and senescence
- Coenzymes and cofactors
- Creatinine
- Inorganic ions
- Intermediary metabolism
- Lipid metabolism
- Nitric oxide
- Phosphates
- Sugars
- Oxidative stress
- Other metabolism assays
-
Apoptosis kits by analyte
- Annexin V
- Calpain
- Caspases
- Cathepsins
- DNA fragmentation
- Transmembrane potential
- Other Apoptosis assays
-
Epigenetic Kits by modification
- DNA modification and methylation
- Histone demethylation
- Histone methylation activity
- Sample Preparation
- Histone methylation quantification
- Histone acetylation and deacetylation
- Histone phosphorylation
- Sumoylation
-
-
Other Kits
-
A-Z
- All kits
-
Antibody labeling
- EasyLink antibody conjugation kits
-
Cell fractionation
- Cell fractionation kits A-Z
-
Cell Staining Kits
- CytoPainter kits
-
-
Biochemicals
A-Z
- By pharmacology
- By research area
- By chemical type
- By product range
Receptors & Transporters
- Adenosine
- Adrenoceptors
- Cannabinoid
- Catalytic Receptors
- Dopamine
- GABA
- Glutamate
- Glycine
- Histamine
- Imidazoline
- Melatonin
- Muscarinic
- Nicotinic
- Nuclear Receptors
- Opioids
- Peptide Receptors
- Purinergics
- Serotonin
- Transporters
-
Immunodetection
-
IHC Kits
- EXPOSE biotin-free detection kits
- Biotin-Streptavidin detection kits
- Endogenous avidin/biotin blocking systems
- Substrate kits
- Mouse on Mouse detection kit
- BrdU IHC detection kit
-
Secondary antibodies
- Advanced search
-
Reagents and buffers
- Buffers
- Chromogens and enhancers
- Diluents
- Epitope recovery and pretreatments
- Fluorescent ligands
- Mounting media
- PAP pen
- Serum
- Substrates
-
-
Controls
-
A-Z
- Isotype controls
- Loading controls
- Lysates
-
Isotype controls by host species
- Chicken
- Goat
- Hamster (Armenian and Syrian)
- Mouse
- Rabbit
- Rat
- Sheep
-
Lysates by type
- 293T transfected
- Cytoplasmic
- Membrane
- Nuclear
- Tissue
- Total protein
- Whole cell
-
Loading controls
- Loading controls by protein target
-
Products:Cell Biology >> Other Antibodies >> Oxidative Stress
Anti-Superoxide Dismutase 3 antibody [1H12] (ab28442)
Product partner
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab28442? Please let us know so that we can cite the reference in this datasheet
ab28442 has been referenced in 1 publications.
- Nightingale H et al. Changes in Expression of the Antioxidant Enzyme SOD3 Occur Upon Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells In Vitro. Stem Cells Dev : (2012). PubMed: 22132904
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - Superoxide Dismutase 3 antibody [1H12] (ab28442)](/ps/datasheet/Images/28/ab28442/Ab28442_1.JPG)
Western blot - Superoxide Dismutase 3 antibody [1H12] (ab28442)
All lanes : Anti-Superoxide Dismutase 3 antibody [1H12] (ab28442)
Lane 1 : Bosc23 cell lysate
Lane 2 : Bosc23 cell lysate transfected with Myc-His-SOD3
Lane 3 : Bosc23 cell lysate transfected with Myc-His-Gpx3
Predicted band size : 26 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
The arrow next to the image points to the Superoxide Dismutase 3 band.
](/ps/datasheet/images/28/ab28442/Superoxide-Dismutase-3-Primary-antibodies-ab28442-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Superoxide Dismutase 3 antibody [1H12](ab28442)
IHC image of ab28442 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28442, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
0
Events
Talk to us
Special offers
© 1998-2012 Abcam plc. All rights reserved.
United States
0
- A - C
- Albania
- Algeria
- Antigua and Barbuda
- Argentina
- Armenia
- Aruba
- Australia
- Austria
- Azerbaijan
- Bahamas
- Bahrain
- Bangladesh
- Barbados
- Belarus
- Belgium
- Belize
- Bermuda
- Bolivia
- Bosnia and herzegovina
- Botswana
- Brazil
- Brunei darussalam
- Bulgaria
- Cambodia
- Cameroon
- Canada
- Cayman islands
- Chile
- China
- Colombia
- Costa rica
- Cote d'ivoire
- Croatia
- Cyprus
- Czech Republic
- D - I
- Denmark
- Dominica
- Dominican republic
- Ecuador
- Egypt
- El salvador
- Estonia
- Ethiopia
- Finland
- France
- French Guiana
- Gabon
- Gambia
- Georgia
- Germany
- Ghana
- Greece
- Grenada
- Guadeloupe
- Guam
- Guatemala
- Guyana
- Haiti
- Honduras
- Hong Kong
- Hungary
- Iceland
- India
- Indonesia
- Ireland
- Israel
- Italy
- J - O
- Jamaica
- Japan
- Jordan
- Kazakhstan
- Kenya
- Korea, Rep of
- Kuwait
- Kyrgystan
- Latvia
- Lebanon
- Lithuania
- Luxembourg
- Macau
- Macedonia
- Madagascar
- Malawi
- Malaysia
- Malta
- Martinique
- Mexico
- Monaco
- Morocco
- Nepal
- Netherlands
- Netherlands Ant
- New caledonia
- New Zealand
- Nicaragua
- Nigeria
- Norway
- Oman
Call 01223 696 000 or contact us