Superoxide Dismutase 3 antibody [1H12] (ab28442)

Product Name 

Superoxide Dismutase 3 antibody [1H12]

Product type 

Primary antibodies

Description 

Mouse monoclonal [1H12] to Superoxide Dismutase 3

Immunogen 

Recombinant full length protein (Human) purified from E.coli.

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
IP: Use at a concentration of 1 µg/ml cell lysate.
WB: 1/2000. Predicted molecular weight: 26 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Bosc23 cell lysate transfected with Myc-His-SOD3.

Cellular localization 

Extracellular. 99% of Superoxide Dismutase 3 is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium.

Research areas 

Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Response to hypoxia
Cell Biology >> Other Antibodies >> Oxidative Stress

Relevance 

Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3). SOD1 is found in all eukaryotic species as a homodimeric 32kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese-containing 80kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin binding multimer of disulfide linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase(CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

1H12

Isotype 

IgG2b

Light chain type 

kappa

Purity 

Protein A purified

Storage buffer 

Preservative: 0.03% Sodium Azide
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

See below for Superoxide Dismutase 3 antibody [1H12] images, references, products related to ab28442 and other tools.

Superoxide Dismutase 3 antibody [1H12] images:

Customer reviews (feedback) regarding Superoxide Dismutase 3 antibody [1H12]

Customer FAQs regarding Superoxide Dismutase 3 antibody [1H12]

Protocols for Superoxide Dismutase 3 antibody [1H12]

Price and availability of products related to Superoxide Dismutase 3 antibody [1H12]

Superoxide Dismutase 3 antibody [1H12] - related products: