![Western blot - Superoxide Dismutase 3 antibody [1H12] (ab28442) image](#Ab28442_1.JPG)
 image](#Superoxide-Dismutase-3-Primary-antibodies-ab28442-1.jpg)
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Products:Cell Biology >> Other Antibodies >> Oxidative Stress
Overview
Product name
Anti-Superoxide Dismutase 3 antibody [1H12]
See all Superoxide Dismutase 3 products (6) ...
Description
Mouse monoclonal [1H12] to Superoxide Dismutase 3
Tested applications
WB, IP, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human) purified from E.coli.
Positive control
Bosc23 cell lysate transfected with Myc-His-SOD3.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.03% Sodium Azide
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
1H12
Isotype
IgG2b
Light chain type
kappa
Research areas
Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Response to hypoxia
Cell Biology >> Other Antibodies >> Oxidative Stress
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000Predicted molecular weight: 26 kDa.
IP: Use at 1 µg/mg of lysate.
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA: Use at an assay dependent dilution.
Target
Function
Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
Tissue specificity
Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.
Sequence similarities
Belongs to the Cu-Zn superoxide dismutase family.
Cellular localization
Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium.
Target information above from: UniProt accessionP08294
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC SOD antibody
- EC-SOD antibody
- Extracellular superoxide dismutase [Cu Zn] antibody
see all
Database links
- Entrez Gene: 6649 Human
- Omim: 185490 Human
- SwissProt: P08294 Human
- SwissProt: Q6FHA2 Human
- Unigene: 2420 Human
Anti-Superoxide Dismutase 3 antibody [1H12] images:
Western blot - Superoxide Dismutase 3 antibody [1H12] (ab28442)
![Western blot - Superoxide Dismutase 3 antibody [1H12] (ab28442)](/ps/datasheet/Images/28/ab28442/Ab28442_1.JPG)
All lanes : Anti-Superoxide Dismutase 3 antibody [1H12] (ab28442)
Lane 1 : Bosc23 cell lysate
Lane 2 : Bosc23 cell lysate transfected with Myc-His-SOD3
Lane 3 : Bosc23 cell lysate transfected with Myc-His-Gpx3
Predicted band size : 26 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
The arrow next to the image points to the Superoxide Dismutase 3 band.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Superoxide Dismutase 3 antibody [1H12](ab28442)
](/ps/datasheet/images/28/ab28442/Superoxide-Dismutase-3-Primary-antibodies-ab28442-1.jpg)
IHC image of ab28442 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28442, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Superoxide Dismutase 3 antibody [1H12] (ab28442)
ab28442 has not yet been referenced specifically in any publications.
Publishing research using ab28442? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"