Products:Cell Biology >> Other Antibodies >> Oxidative Stress
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ab80946 at 1/100 dilution staining Superoxide Dismutase 3 in human cartilage by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A HRP-conjugated anti mouse secondary was used at 1/10 dilution. Image 'a' repersents control, 'b' staining of tissue from young human cartilage and 'c' show staining cartilage of a person suffered from osteoarthritis.
Anti-Superoxide Dismutase 3 antibody [4G11G6] (ab80946) at 1 µg/ml + Lung (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 26 kDa
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
Additional bands at : 54 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
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