![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Superoxide Dismutase 3 antibody [4G11G6] (ab80946) image](#Superoxide-Dismutase-3-Primary-antibodies-ab80946-10.jpg)
![Western blot - Superoxide Dismutase 3 antibody [4G11G6] (ab80946) image](#Superoxide-Dismutase-3-Primary-antibodies-ab80946-12.jpg)
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Products:Cell Biology >> Other Antibodies >> Oxidative Stress
Overview
Product name
Anti-Superoxide Dismutase 3 antibody [4G11G6]
See all Superoxide Dismutase 3 products (6) ...
Description
Mouse monoclonal [4G11G6] to Superoxide Dismutase 3
Specificity
Detects extracellular Superoxide Dismutase 3
Tested applications
ELISA, ICC/IF, WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Full length native Human protein purified from aortas
Positive control
Human cartilage lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
4G11G6
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Response to hypoxia
Cell Biology >> Other Antibodies >> Oxidative Stress
Applications
Show applications key
Our Abpromise guarantee covers the use of ab80946 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
WB: 1/1000Detects a band of approximately 35 kDa (predicted molecular weight: 26 kDa).
IHC-P: 1/100
IHC-Fr: Use at an assay dependent dilution.
Target
Function
Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
Tissue specificity
Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.
Sequence similarities
Belongs to the Cu-Zn superoxide dismutase family.
Cellular localization
Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium.
Target information above from: UniProt accessionP08294
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC SOD antibody
- EC-SOD antibody
- Extracellular superoxide dismutase [Cu Zn] antibody
see all
Database links
- Entrez Gene: 6649 Human
- Entrez Gene: 20657 Mouse
- Entrez Gene: 25352 Rat
- Omim: 185490 Human
- SwissProt: P08294 Human
- SwissProt: Q6FHA2 Human
- SwissProt: O09164 Mouse
- SwissProt: O88592 Mouse
see all
Anti-Superoxide Dismutase 3 antibody [4G11G6] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Superoxide Dismutase 3 antibody [4G11G6] (ab80946)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Superoxide Dismutase 3 antibody [4G11G6] (ab80946)](/ps/datasheet/images/80/ab80946/Superoxide-Dismutase-3-Primary-antibodies-ab80946-10.jpg)
ab80946 at 1/100 dilution staining Superoxide Dismutase 3 in human cartilage by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A HRP-conjugated anti mouse secondary was used at 1/10 dilution. Image 'a' repersents control, 'b' staining of tissue from young human cartilage and 'c' show staining cartilage of a person suffered from osteoarthritis.
Western blot - Superoxide Dismutase 3 antibody [4G11G6] (ab80946)
![Western blot - Superoxide Dismutase 3 antibody [4G11G6] (ab80946)](/ps/datasheet/images/80/ab80946/Superoxide-Dismutase-3-Primary-antibodies-ab80946-12.jpg)
Anti-Superoxide Dismutase 3 antibody [4G11G6] (ab80946) at 1 µg/ml + Lung (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 26 kDa
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
Additional bands at : 54 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
References for Anti-Superoxide Dismutase 3 antibody [4G11G6] (ab80946)
This product has been referenced in:
- Regan Eet al. Extracellular superoxide dismutase and oxidant damage in osteoarthritis. Arthritis Rheum 52:3479-91 (2005). ELISA; Human.Read more (PubMed: 16255039) »
See 1 publication for this product
Publishing research using ab80946? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"