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Products:Cell Biology >> Other Antibodies >> Oxidative Stress
Anti-Superoxide Dismutase 3 antibody (ab21974)
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Secondary antibodies
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Immunohistochemistry (Frozen sections) - Superoxide Dismutase 3 antibody (ab21974)
ab21974 (1/1000) staining of ciliated bronchiolar cells in human lung.
Immunohistochemistry (Frozen sections) - Superoxide Dismutase 3 antibody (ab21974)
ab21974 (1/1000) staining of human kidney tubule cells.
Western blot - Superoxide Dismutase 3 antibody (ab21974)
All lanes : Anti-Superoxide Dismutase 3 antibody (ab21974) at 1/500 dilution
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 :
Lane 3 :
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 26 kDa
Observed band size : 31 kDa (why is the actual band size different from the predicted?)
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
Superoxide Dismutase 3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Superoxide Dismutase 3 antibody for Western blot in Mouse (21974)
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