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Products:Cell Biology >> Other Antibodies >> Oxidative Stress
Overview
Product name
Anti-Superoxide Dismutase 3 antibody
See all Superoxide Dismutase 3 products (6) ...
Description
Rabbit polyclonal to Superoxide Dismutase 3
Specificity
Specific to human extracellular SOD (SOD3). Under reducing conditions the SOD3 antibody shows a major band at approximately 30kDa which is the approximate molecular weight of the SOD3 monomer and two faint minor bands at approximately 50 and 70 kDa. The SOD3 molecule is a homotetramer (4 monomers) with a combined molecular weight of around a 130 Kda. The two faint bands are probably dimers and triplicates of the monomer. This pattern is common with many tetramers (such as cellular glutathione peroxidase) as there is incomplete reduction with DTT.
Tested applications
IHC-Fr, IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: WTGEDSAEPNSDSAEWIRD, corresponding to N terminal amino acids 19-37 of Human Superoxide Dismutase 3.
WTGEDSAEPN SDSAEWIRD
Positive control
IHC: cortex of Dementia patient with Lewy bodies WB: human lung lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Response to hypoxia
Cell Biology >> Other Antibodies >> Oxidative Stress
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/200 - 1/2000.
IHC-P: 1/200 - 1/2000.(Antigen retrieval has been used but may not be necessary.)
WB: 1/1000 - 1/4000.Detects a band of approximately 31 kDa (predicted molecular weight: 26 kDa).
Target
Function
Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
Tissue specificity
Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.
Sequence similarities
Belongs to the Cu-Zn superoxide dismutase family.
Cellular localization
Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium.
Target information above from: UniProt accessionP08294
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ALS antibody
- ALS1 antibody
- Cu/Zn superoxide dismutase antibody
see all
Database links
- Entrez Gene: 6649 Human
- Omim: 185490 Human
- SwissProt: P08294 Human
- SwissProt: Q6FHA2 Human
- Unigene: 2420 Human
Anti-Superoxide Dismutase 3 antibody images:
Immunohistochemistry (Frozen sections) - Superoxide Dismutase 3 antibody (ab21974)
ab21974 (1/1000) staining of ciliated bronchiolar cells in human lung.
Immunohistochemistry (Frozen sections) - Superoxide Dismutase 3 antibody (ab21974)
ab21974 (1/1000) staining of human kidney tubule cells.
Western blot - Superoxide Dismutase 3 antibody (ab21974)
All lanes : Anti-Superoxide Dismutase 3 antibody (ab21974) at 1/500 dilution
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)
Lane 3 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 26 kDa
Observed band size : 31 kDa (why is the actual band size different from the predicted?)
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
Superoxide Dismutase 3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
References for Anti-Superoxide Dismutase 3 antibody (ab21974)
This product has been referenced in:
- Mital Ret al. Antioxidant network expression abrogates oxidative posttranslational modifications in mice. Am J Physiol Heart Circ Physiol 300:H1960-70 (2011).Read more (PubMed: 21335461) »
- Moningka NCet al. Protective actions of nebivolol on chronic nitric oxide synthase inhibition-induced hypertension and chronic kidney disease in the rat: a comparison with angiotensin II receptor blockade. Nephrol Dial Transplant : (2011).Read more (PubMed: 21856762) »
See all 3 publications for this product
Publishing research using ab21974? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"