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Superoxide Dismutase Activity Colorimetric Assay Kit (ab65354)

Q&A (34)Specific References (4)

Overview

  • Product nameSuperoxide Dismutase Activity Colorimetric Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeEnzyme activity
  • Assay time
    0h 30m
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human, Pig
    Predicted to work with: all Mammals
  • Product overview

    Abcam's Superoxide Dismutase Activity Colorimetric Assay Kit is a sensitive kit using WST-1 that produces a water-soluble formazan dye upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD. Therefore, the inhibition activity of SOD can be determined by a colorimetric method.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Superoxide dismutase (SOD) is one of the most important antioxidative enzymes. It catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceSuperoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3).
  • Cellular localizationCytoplasmic
    • Alternative names
        ALSALS1Cu /Zn superoxide dismutase
        Cu/Zn superoxide dismutaseIPOASODSOD solubleSOD1Superoxide dismutase 1 solubleSuperoxide dismutase cystolic
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab65354 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Functional Studies FuncS

      Superoxide Dismutase Activity Colorimetric Assay Kit images

      • Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)
        Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)
        Principle of Superoxide Dismutase Assay.

      Protocols

      References for Superoxide Dismutase Activity Colorimetric Assay Kit (ab65354)

      This product has been referenced in:
      • Ioannou A  et al. Complement depletion protects lupus-prone mice from ischemia-reperfusion-initiated organ injury. Am J Physiol Gastrointest Liver Physiol 304:G283-92 (2013). Read more (PubMed: 23104558) »
      • Khan M  et al. Growth factor preconditioning increases the function of diabetes-impaired mesenchymal stem cells. Stem Cells Dev 20:67-75 (2011). Read more (PubMed: 20446810) »

      See all 4 Publications for this product

      Publishing research using ab65354 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

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      Displaying 1 - 10 of 34 results for Abreviews and Q&A

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      Inquiry: Does this Sod assay kit also work for prokaryotic cells? Pseudomonas aeruginosa PAO1? Thanks

      Thank you for contacting us.

      This kit has been tested in human samples only, so it may work for prokaryotic cells but we have no data on testing these types of samples.

      I hope this information is helpful to you. Please do not hesitate to...

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      How much tissue should be used for extraction?

      We would recommend using at least 10 mg of tissue with the kit ab65354.

      Customer called to inquire about the standard for this product.

      We recommend use of ab112193 SOD protein as standard for this kit.

      Is measuring protein concentration appropriate with this kit?
      If I want to load equal protein levels in all wells, and some samples have different concentrations of protein, should I adjust the concentrations before adding protein to the well so that...

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      It is recommended to add the same amount of protein into each well. For that you can use the SOD assay buffer for bringing up the volume in each well.

      Are three blanks required for each sample, or can three blanks suffice for comparison to all samples run at the same time?

      Yes, you would need the 3 controls with each and every sample.

      ab65354 Superoxide Dismutase Activity Colorimetric Assay Kit

      Can you recommend RBC lysis buffer for human to this product?

      J62990 RBC lysis buffer for human (Applications: For lysis of human red blood cells.) is good / compatible wit...

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      Thank you for your enquiry and interest in our products.

      The Protocol Booklet for using RBCs/erythrocytes is mentioned in the datasheet for this kit, as such:

      Blood samples: Collect blood using citrate or EDTA. Centrifuge at 1,000 x g ...

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      Dear sir, madam;

      We bought the superoxide dismutase activity assay kit catalog number ab65354. In the kit, no SOD standard has been supplied, therefore we would like to buy one. Can we use ab73795 active SOD protein for this purpose?

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      Thank you for your enquiry.

      I have investigated this and can confirm that we would recommend to use ab112193 SOD protein if you need to use a standard with the ab65354 kit. I can recommend to review the online datasheet for further information:...

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      I am interested in your Catalase and Superoxid Dismutase Activity Assay Kits. Is it possible to use frozen tissue for the assay (stored at -70°C)?

      Vielen Dank für Ihre Anfrage.

      Laut unserem Labor ist es völlig in Ordnung, wenn Sie gefrorenes Material verwenden, da wir dies teilweise auch in unserem Labor verwenden.

      Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie...

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      We have purchased 2 SOD testing kits ( ab 65354) from your company. These kits do
      not contain standards. Can you provide us the SOD standard?

      Thank you for contacting us.

      As indicated in the protocol booklet and on the datasheet, this kit does indeed not include the SOD standard.

      However, as a standard, your client may want to use ab90040 Superoxide Dismutase 1 protein (Act...

      Read More


      I have a question regarding your product Superoxide Dismutase Activity Colorimetric Assay Kit (ab65354). Would this assay work on frozen tissue samples.

      Since this is an enzymatic assay it depends on how well the tissue was frozen. There is a chance that the SOD might have degraded during the process of freezing and if so, this assay will not work on such frozen tissue samples. Here are some references fo...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"