Superoxide Dismutase Activity Colorimetric Assay Kit (ab65354)
- Product nameSuperoxide Dismutase Activity Colorimetric Assay Kit
- Tests100 x 1 assay
- Sample typeUrine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
- Assay typeEnzyme activity
- Assay time0h 30m
- Species reactivityReacts with: Mouse, Rat, Sheep, Human, Pig
Predicted to work with: all Mammals
- Product overview
Abcam's Superoxide Dismutase Activity Colorimetric Assay Kit is a sensitive kit using WST-1 that produces a water-soluble formazan dye upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD. Therefore, the inhibition activity of SOD can be determined by a colorimetric method.
Visit our FAQs page for tips and troubleshooting.
Superoxide dismutase (SOD) is one of the most important antioxidative enzymes. It catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen.
- Tested applicationsFunctional Studies more details
Components Identifier 100 tests SOD Assay Buffer WM 1 x 20ml SOD Dilution Buffer NM 1 x 10ml SOD Enzyme Solution Green 1 x 20µl WST Solution Red 1 x 1ml
- Research Areas
- ALSALS1Cu /Zn superoxide dismutase
- Cu/Zn superoxide dismutaseIPOASODSOD solubleSOD1Superoxide dismutase 1 solubleSuperoxide dismutase cystolic
Our Abpromise guarantee covers the use of ab65354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Superoxide Dismutase Activity Colorimetric Assay Kit images
Principle of Superoxide Dismutase Assay.
References for Superoxide Dismutase Activity Colorimetric Assay Kit (ab65354)
This product has been referenced in:
- Ioannou A et al. Complement depletion protects lupus-prone mice from ischemia-reperfusion-initiated organ injury. Am J Physiol Gastrointest Liver Physiol 304:G283-92 (2013). Read more (PubMed: 23104558) »
- Khan M et al. Growth factor preconditioning increases the function of diabetes-impaired mesenchymal stem cells. Stem Cells Dev 20:67-75 (2011). Read more (PubMed: 20446810) »