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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab28083? Please let us know so that we can cite the reference in this datasheet
ab28083 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Suppressor of Fused antibody (ab28083)
All lanes : Anti-Suppressor of Fused antibody (ab28083) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Lane 3 :
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 54 kDa
Additional bands at : 37 kDa (possible cleavage fragment,cross reactivity).
Immunocytochemistry/ Immunofluorescence - Suppressor of Fused antibody (ab28083)
ICC/IF image of ab28083 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab28083, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Western blot - Suppressor of Fused antibody (ab28083)
Anti-Suppressor of Fused antibody (ab28083) at 1 µg/ml + Testis (Mouse) Tissue Lysate - normal tissue at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 54 kDa
Anti-Suppressor of Fused antibody for Western blot in Mouse (28083)
Suppressor of Fused antibody for Western blot in Human (28083)
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