Products:Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
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Has this Ab against SUFU been tested in IHC apllications for parrafin or frozen sections? Thanks
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ANSWER: |
Thank you for your enquiry. This antibody has only been tested in Western Blot. That does not mean that it won't work in IHC applications, it just means that we haven't tested it and therefore can not guarantee that it will work in those applications. If you choose to purchase the antibody and use it for IHC, I encourage you to let us know how it goes by submitting an Abreview. By submitting Abreviews, you can earn Abpoints which can be used towards various promotions such as Amazon.com gift certificates or a future antibody purchase. I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-Suppressor of Fused antibody (ab28083) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Lane 3 :
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 54 kDa
Additional bands at : 37 kDa (possible cleavage fragment,cross reactivity).
ICC/IF image of ab28083 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab28083, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Anti-Suppressor of Fused antibody (ab28083) at 1 µg/ml + Testis (Mouse) Tissue Lysate - normal tissue at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 54 kDa
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