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Western blot - Surf1 antibody (ab66003)
(enlarge)
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Product Name
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Surf1 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Surf1
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Immunogen
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A synthesized peptide derived from the internal region of human Surf1.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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This antibody detects endogenous levels of total Surf1 protein.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/20000.
WB: 1/500 - 1/1000. Detects bands of approximately 33, 80 and 116 kDa (predicted molecular weight: 33 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from Jurkat cells
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Cellular localization
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Mitochondrion inner membrane; Multipass membrane protein
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Research areas
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Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
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Relevance
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Surf1 is thought to be involved in the biogenesis of the cytochrome c oxidase complex. The gene is located in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity, where it shares a bidirectional promoter with Surf2 on the opposite strand. Defects in this gene are a cause of Leigh syndrome, a severe neurological disorder that is commonly associated with systemic cytochrome c oxidase deficiency.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg+2 and Ca+2), 150mM Sodium chloride, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Surf1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Surf1 antibody images, references, products related to ab66003 and other tools.
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Surf1 antibody images:
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 Western blot - Surf1 antibody (ab66003)
All lanes : Surf1 antibody (ab66003) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells at 5-30ug
Lane 2 : Extracts from Jurkat cells at 5-30ug with immunizing peptide at 5-10ug
Predicted band size : 33 kDa
Observed band size : 33 kDa
Additional bands at : 116 kDa,80 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to Surf1
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Surf1 antibody - more information
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Customer reviews (feedback) regarding Surf1 antibody |
Customer FAQs regarding Surf1 antibody |
Protocols for Surf1 antibody |
Price and availability of products related to Surf1 antibody |
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Surf1 antibody - related products:
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Surf1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Surf1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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