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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Overview
Product name
Anti-Surf1 antibody
See all Surf1 products (3) ...
Description
Rabbit polyclonal to Surf1
Specificity
This antibody detects endogenous levels of total Surf1 protein.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
A synthesized peptide derived from the internal region of human Surf1.
Positive control
Extracts from Jurkat cells
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg+2 and Ca+2), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
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Western blot - Surf1 antibody (ab66003)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
Application notes
ELISA: 1/20000.
WB: 1/500 - 1/1000. Detects bands of approximately 33, 80 and 116 kDa (predicted molecular weight: 33 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Probably involved in the biogenesis of the COX complex.
Involvement in disease
Defects in SURF1 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions that is commonly associated with systemic cytochrome c oxidase (COX) deficiency.
Sequence similarities
Belongs to the SURF1 family.
Cellular localization
Mitochondrion inner membrane.
Target information above from: UniProt accessionQ15526
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- OTTHUMP00000022473 antibody
- OTTHUMP00000022474 antibody
- SURF 1 antibody
see all
Database links
- Entrez Gene: 6834 Human
- Entrez Gene: 20930 Mouse
- Entrez Gene: 64463 Rat
- Omim: 185620 Human
- SwissProt: Q15526 Human
- SwissProt: P09925 Mouse
- SwissProt: Q9QXU2 Rat
- Unigene: 512464 Human
see all
Anti-Surf1 antibody images:
Western blot - Surf1 antibody (ab66003)
All lanes : Anti-Surf1 antibody (ab66003) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells at 5-30ug
Lane 2 : Extracts from Jurkat cells at 5-30ug with immunizing peptide at 5-10ug
Predicted band size : 33 kDa
Observed band size : 33 kDa
Additional bands at : 116 kDa,80 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Surf1 antibody (ab66003)
ab66003 has not yet been referenced specifically in any publications.
Publishing research using ab66003? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"