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Products:Cardiovascular >> Blood >> Other

Anti-Surfactant Protein B (Mature) antibody [SPB02 (same as 1B9)] (ab3282)

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Overview

Product name

Anti-Surfactant Protein B (Mature) antibody [SPB02 (same as 1B9)]
See all Surfactant Protein B (Mature) products (4) ...

Description

Mouse monoclonal [SPB02 (same as 1B9)] to Surfactant Protein B (Mature)

Tested applications

IHC-Fr, IF, IHC-P, WBmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Recombinant full length protein.

Positive control

Lung adenocarcinomas.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide

Concentration

Concentration information loading...

Purity

Protein A purified

Primary antibody notes

ProSPB is glycosylated in the Golgi apparatus and undergoes carboxy and amino terminal proteolysis by a cathepsin D like protease. Pulmonary surfactant is a complex mixture of phospholipids and proteins that is secreted from type II cells in alveoli and reduces the surface tension atthe alveolar air liquid interface, providing alveolar stability necessary for normal ventillation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C and D. SP A (28 - 36 kDa) and SP D (43 kDa) are collagenous carbohydrate binding proteins, whereas SP B (8 - 9 kDa) and SP C (4 kDa) are non collagenous hydrophobic proteins. SP B is expressed in pulmonary adenocarcinomas with acinar, papillary, bronchioloalveolar, and solid growth patterns. Squamous cell and large cell carcinomas of the lung and nonpulmonary adenocarcinomas do not express SP B.

Clonality

Monoclonal

Clone number

SPB02 (same as 1B9)

Isotype

IgG2a

Light chain type

kappa

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Lipases
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Lipases
Cardiovascular >> Blood >> Other

  • Immunohistochemistry (Frozen sections) - Surfactant Protein B (Mature) antibody [SPB02 (same as 1B9)] (ab3282)Immunohistochemistry (Frozen sections) - Surfactant Protein B (Mature) antibody [SPB02 (same as 1B9)] (ab3282) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

Involvement in disease

Defects in SFTPB are the cause of pulmonary surfactant metabolism dysfunction type 1 (SMDP1) [MIM:265120]; also called pulmonary alveolar proteinosis due to surfactant protein B deficiency. A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
Genetic variations in SFTPB are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS) [MIM:267450]. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'. Note=A variation Ile to Thr at position 131 influences the association between specific alleles of SFTPA1 and respiratory distress syndrome in premature infants.

Sequence similarities

Contains 1 saposin A-type domain.
Contains 3 saposin B-type domains.

Cellular localization

Secreted > extracellular space > surface film.

Target information above from: UniProt accessionP07988 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 18 kDa pulmonary surfactant protein antibody
  • 18 kDa pulmonary-surfactant protein antibody
  • 6 kDa protein antibody
  • PSPB antibody
  • PSPB_HUMAN antibody
  • Pulmonary surfactant apoprotein PSP B antibody
  • Pulmonary surfactant associated protein B 18kD antibody
  • Pulmonary surfactant associated protein B antibody
  • Pulmonary surfactant associated proteolipid SPL Phe antibody
  • Pulmonary surfactant-associated protein B antibody
  • Pulmonary surfactant-associated proteolipid SPL(Phe) antibody
  • SFTB3 antibody
  • SFTP3 antibody
  • SFTPB antibody
  • SFTPB surfactant antibody
  • SP B antibody
  • SP-B antibody
  • SPB antibody
  • Surfactant pulmonary associated protein B antibody
see all

Database links

Anti-Surfactant Protein B (Mature) antibody [SPB02 (same as 1B9)] images:

  Immunohistochemistry (Frozen sections) - Surfactant Protein B (Mature) antibody [SPB02 (same as 1B9)] (ab3282)

Immunohistochemistry (Frozen sections) - Surfactant Protein B (Mature) antibody [SPB02 (same as 1B9)] (ab3282)

ab3282 at a 1/100 dilution staining Surfactant Protein B (Mature) from Mouse Tissue sections (Lung) by Immunohistochemistry (Frozen sections). The antibody was incubated with the tissue for 24 hours and then detected using an Alexa Fluor® 488 Goat anti-mouse IgG antibody.

This image is courtesy of an Abreview submitted by an ananymous researcher on 5 January 2006.

See Abreview

References for Anti-Surfactant Protein B (Mature) antibody [SPB02 (same as 1B9)] (ab3282)

This product has been referenced in:

See all 2 publications for this product

Publishing research using ab3282? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"