Survivin 2B antibody (ab3729)

Product Name 

Survivin 2B antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Survivin 2B

Immunogen 

Synthetic peptide derived from residues 50 - 150 of Human Survivin 2B.

Reacts with 
(species key)

Hu

Specificity 

This antibody has been tested in Western blotting against equal loadings of purified wild type survivin, 2B and DEx3. When used at 1/500, it detects a single clean band of the appropriate size for 2B, but no band is detected with wild type survivin or DEx3.

We have not succeeded in detecting endogenous Survivin 2B using this antibody (Survivin 2B is expressed at very low levels).

This antibody has been used successfully however, to detect overexpressed Survivin 2B in whole cell extracts.

Tested applications 
(see key)

ICC/IF, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent concentration.
IF: 1/50.
WB: 1/500. Found to work at 1/5000 dilution (see PMID: 16847456). Predicted molecular weight: 18 kDa. Can be blocked with Survivin 2B peptide (ab13762).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear (chromossome arms and centromeres) during prophase and metaphase; Cytoplasmic (mitotic spindle) during anaphase and telophase.

Research areas 

Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Inhibitors
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs

Relevance 

Survivin, a new inhibitor of apoptosis (IAP) protein, is expressed in the G2 / M phase of the cell cycle in a cycle regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase 3 activity, a mechanism involved in cell death, during mitosis. It indicates that survivin may counteract a default induction of apoptosis in G2 / M phase. The over-expression of survivin in cancer may overcome this apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis.
Survivin DEx3 (lacking exon 3) and survivin-2B (retaining a part of intron 2 as a cryptic exon) are isoforms of survivin that behave differently to survivin. Survivin-2B has acquired additional 23 amino acids encoded by the cryptic exon 2B. See Mahotka et al. 1999 for more details.Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA. pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 0.12 - 0.40 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

This antibody should be aliquoted to reduce freeze-thaw cycles as it loses performance quickly after multiple freeze-thaws.

References for Survivin 2B antibody (ab3729)

  This product has been used in: (two most recent references)

Small TW & Pickering JG Nuclear degradation of Wilms tumor 1-associating protein and survivin splice variant switching underlie IGF-1-mediated survival. J Biol Chem 284:24684-95 (2009). WB; Human. PubMed: 19605357

V gran F et al. Association of p53 gene alterations with the expression of antiapoptotic survivin splice variants in breast cancer. Oncogene 26:290-7 (2007). PubMed: 16847456

See all 4 publication references for this product.


If you publish research using ab3729 please let us know so that we can cite the reference on this datasheet.

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