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Read our guarantee »Products:Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Anti-Survivin 2B antibody
Rabbit polyclonal to Survivin 2B
ICC/IF, WB, IFmore details
Reacts with
Human
Synthetic peptide derived from residues 50 - 150 of Human Survivin 2B.
This antibody gave a positive siginal in Jurkat whole cell lysate.
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Inhibitors
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Western blot - Survivin 2B antibody (ab3729)
(enlarge)
Our Abpromise guarantee covers the use of ab3729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).Can be blocked with Survivin 2B peptide (ab13762).
IF: 1/50
Survivin, a new inhibitor of apoptosis (IAP) protein, is expressed in the G2 / M phase of the cell cycle in a cycle regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase 3 activity, a mechanism involved in cell death, during mitosis. It indicates that survivin may counteract a default induction of apoptosis in G2 / M phase. The over-expression of survivin in cancer may overcome this apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis. Survivin DEx3 (lacking exon 3) and survivin-2B (retaining a part of intron 2 as a cryptic exon) are isoforms of survivin that behave differently to survivin. Survivin-2B has acquired additional 23 amino acids encoded by the cryptic exon 2B. See Mahotka et al. 1999 for more details.Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas.
Nuclear (chromossome arms and centromeres) during prophase and metaphase; Cytoplasmic (mitotic spindle) during anaphase and telophase.
Western blot - Survivin 2B antibody (ab3729)

Anti-Survivin 2B antibody (ab3729) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 18 kDa
Observed band size : 16 kDa (why is the actual band size different from the predicted?)
Additional bands at : 13 kDa,37 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 5 minutes
This product has been referenced in:
See all 4 publications for this product
Publishing research using ab3729? Please let us know so that we can cite the reference in this datasheet
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Anti-Survivin 2B antibody (ab3729) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 18 kDa
Observed band size : 16 kDa (why is the actual band size different from the predicted?)
Additional bands at : 13 kDa,37 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 5 minutes
1
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