Overview
- Product nameSurvivin Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
- Assay typeSandwich
- Sensitivity< 2 pg/ml
- Range62.5 pg/ml - 4000 pg/ml
- Species reactivityReacts with: Human
- Product overview
ab119607 Survivin Human ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human Survivin specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Survivin amount of sample captured in plate.
No detectable cross-reactivity with any other cytokine.
- Notes
Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 96-well plate precoated with anti-human Survivin antibody (12 strips) 1 unit ABC diluent buffer 1 x 12ml Antibody diluent buffer 1 x 12ml Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-human Survivin antibody 1 x 130µl Lyophilized recombinant human Survivin standard 2 vials Sample diluent buffer 1 x 30ml TMB color developing agent 1 x 10ml TMB stop solution 1 x 10ml -
Research Areas
Contains 1 BIR repeat.
modificationsUbiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
Target information above from: UniProt accession
O15392
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- API4Apoptosis inhibitor 4Apoptosis inhibitor survivin
- Baculoviral IAP repeat containing 5Baculoviral IAP repeat-containing protein 5BIRC5BIRC5_HUMANEPR 1
see all
- Entrez Gene: 332 Human
- Omim: 603352 Human
- SwissProt: O15392 Human
- Unigene: 514527 Human
Applications
Our Abpromise guarantee covers the use of ab119607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
Survivin Human ELISA Kit images
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Sandwich ELISA - Survivin Human ELISA Kit (ab119607)Representative standard curve using ab119607.
Protocols
References for Survivin Human ELISA Kit (ab119607)
ab119607 has not yet been referenced specifically in any publications.
