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Overlay histogram showing Human Colo 205 cells stained with ab3258 (unshaded). Cells were prepared with trypsinization. The cells were fixed with paraformaldehyde and then permeabilized with 70% methanol. The antibody was diluted 1/200 in PBS and incubated with the sample for one hour at 22ºC. The secondary antibody used was FITC Rabbit anti-Mouse polyclonal at 1/100 dilution. The isotype control (shaded histogram) was used under the same conditions.
This image is courtesy of an Anonymous Abreview.
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