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Read our guarantee »Products:Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Anti-Survivin antibody [8E2]
See all Survivin products (18) ...
Mouse monoclonal [8E2] to Survivin
Flow Cyt, ICC/IF, IHC-Pmore details
Reacts with
Rat, Human
Recombinant full length protein (Human).
Amino acids 1 - 99.
HeLa cells.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
Concentration information loading...
Protein G purified
Monoclonal
8E2
IgG1
kappa
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Flow Cytometry - Survivin antibody [8E2] (ab3258)
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Our Abpromise guarantee covers the use of ab3258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent dilution. (Used a dilution of 1/200 for 2 hrs on human prostate cancer cells (see Abreview submitted on 22 June 2006).)
IHC-P: Use a concentration of 2 - 4 µg/ml.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Is unsuitable for or WB.
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
Belongs to the IAP family.
Contains 1 BIR repeat.
Expression is cell cycle-dependent and peaks at mitosis.
The BIR repeat is necessary and sufficient for HBXIP binding.
Ubiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
Target information above from: UniProt accessionO15392
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Flow Cytometry - Survivin antibody [8E2] (ab3258)
![Flow Cytometry - Survivin antibody [8E2] (ab3258)](/ps/datasheet/images/3/ab3258/Survivin-Primary-antibodies-ab3258-3.jpg)
Overlay histogram showing Human Colo 205 cells stained with ab3258 (unshaded). Cells were prepared with trypsinization. The cells were fixed with paraformaldehyde and then permeabilized with 70% methanol. The antibody was diluted 1/200 in PBS and incubated with the sample for one hour at 22ºC. The secondary antibody used was FITC Rabbit anti-Mouse polyclonal at 1/100 dilution. The isotype control (shaded histogram) was used under the same conditions.
This image is courtesy of an Anonymous Abreview.
ab3258 has not yet been referenced specifically in any publications.
Publishing research using ab3258? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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![Flow Cytometry - Survivin antibody [8E2] (ab3258)](/ps/datasheet/images/3/ab3258/Survivin-Primary-antibodies-ab3258-3.jpg)
Overlay histogram showing Human Colo 205 cells stained with ab3258 (unshaded). Cells were prepared with trypsinization. The cells were fixed with paraformaldehyde and then permeabilized with 70% methanol. The antibody was diluted 1/200 in PBS and incubated with the sample for one hour at 22ºC. The secondary antibody used was FITC Rabbit anti-Mouse polyclonal at 1/100 dilution. The isotype control (shaded histogram) was used under the same conditions.
This image is courtesy of an Anonymous Abreview.
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