|
|
|
|
|
Product Name
|
Survivin antibody [8E2]
|
| |
See all Survivin antibodies (20)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [8E2] to Survivin
|
|
Immunogen
|
Recombinant full length protein (Human).
|
|
Reacts with
(species key)
|
Hu, Rat
|
|
Epitope
|
Amino acids 1 - 99.
|
|
Tested applications
(see key)
|
ICC/IF, IHC-P
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IF: Used a dilution of 1/200 for 2 hrs on human prostate cancer cells (see Abreview submitted on 22 June 2006).
IHC-P: Use at a concentration of 2 - 4 µg/ml for 30 mins at RT. Require boiling tissue in 10 mM citrate buffer for 10-20 mins. followed by cooling at RT 10-20 mins.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
HeLa cells.
|
|
Cellular localization
|
Cytoplasmic and Nuclear
|
|
Research areas
|
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
|
|
Relevance
|
Survivin is an anti-apoptotic protein, expressed in the G2/M cell cycle phase. At the onset of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptotic function and increased caspase 3 activity.
Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein. Survivin might affect regulation of neural cell proliferative responses after brain injury. Survivin is also an inhibitor of apoptosis (IAP), or programmed cell death, that is selectively overexpressed in common human cancers.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
8E2
|
|
Isotype
|
IgG1
|
|
Light chain type
|
kappa
|
|
Purity
|
Protein G purified
|
|
Storage buffer
|
10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C. Do not freeze.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Survivin antibody [8E2] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
|
Search PubMed (MEDLINE) for references to Survivin
|
| |
Survivin antibody [8E2] - more information
|
Customer reviews (feedback) regarding Survivin antibody [8E2] |
Customer FAQs regarding Survivin antibody [8E2] |
Protocols for Survivin antibody [8E2] |
Price and availability of products related to Survivin antibody [8E2] |
| |
Survivin antibody [8E2] - related products:
|
|
|
| |
Survivin antibody [8E2] - other tools:
|
Contact Abcam with a Technical Enquiry about Survivin antibody [8E2] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
