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Product Name 

Survivin antibody [8E2]

 

See all Survivin antibodies (20)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [8E2] to Survivin

Immunogen 

Recombinant full length protein (Human).

Reacts with 
(species key)

Hu, Rat

Epitope 

Amino acids 1 - 99.

Tested applications 
(see key)

ICC/IF, IHC-P


Abreviews 

Customer reviews
(see key)


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• ICC/IF

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Application notes 
(see key)

Recommended dilutions
IF: Used a dilution of 1/200 for 2 hrs on human prostate cancer cells (see Abreview submitted on 22 June 2006).
IHC-P: Use at a concentration of 2 - 4 µg/ml for 30 mins at RT. Require boiling tissue in 10 mM citrate buffer for 10-20 mins. followed by cooling at RT 10-20 mins.


Is unsuitable for WB.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa cells.

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs

Relevance 

Survivin is an anti-apoptotic protein, expressed in the G2/M cell cycle phase. At the onset of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptotic function and increased caspase 3 activity.
Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein. Survivin might affect regulation of neural cell proliferative responses after brain injury. Survivin is also an inhibitor of apoptosis (IAP), or programmed cell death, that is selectively overexpressed in common human cancers.

 

Alternative names Survivin antibody [8E2] (ab3258)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

332    (Human)
64041    (Rat)

Omim

    

603352    (Human)

SwissProt

    

O15392    (Human)
Q9JHY7    (Rat)

Unigene

    

514527    (Human)
54471    (Rat)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

8E2

Isotype 

IgG1

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Survivin antibody [8E2] is on this datasheet. But please do contact us if you would like any reassurance!


 

Search PubMed (MEDLINE) for references to Survivin

 

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Survivin antibody [8E2] - other tools:

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