Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Proteins and Peptides
- Secondary antibodies
- Lysates
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-Survivin antibody
See all Survivin products (18) ...
Description
Rabbit polyclonal to Survivin
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide, corresponding to amino acids 1-12 of Human Survivin.
Positive control
Molt4 whole cell lysate, Raji and Jurkat lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Western Blot at 0.5 to 1 µg/ml gives a band at 17 kD.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
Tissue specificity
Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
Sequence similarities
Belongs to the IAP family.
Contains 1 BIR repeat.
Developmental stage
Expression is cell cycle-dependent and peaks at mitosis.
Domain
The BIR repeat is necessary and sufficient for HBXIP binding.
Post-translational
modifications
Ubiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
Cellular localization
Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
Target information above from: UniProt accessionO15392
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- API4 antibody
- Apoptosis Inhibitor 4 antibody
- Apoptosis inhibitor survivin antibody
see all
Database links
- Entrez Gene: 332 Human
- Omim: 603352 Human
- SwissProt: O15392 Human
- Unigene: 514527 Human
Anti-Survivin antibody images:
Western blot - Survivin antibody (ab2050)
All lanes : Anti-Survivin antibody (ab2050) at 1 µg/ml
Lane 1 : MOLT4 cell lysate with absence of blocking peptide
Lane 2 : MOLT4 cell lysate with presence of blocking peptide
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Survivin antibody (ab2050)
ab2050 at 5µg/ml staining Survivin in Jurkat cells by ICC/IF
Protocols
References for Anti-Survivin antibody (ab2050)
ab2050 has not yet been referenced specifically in any publications.
Publishing research using ab2050? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"