Overview

  • Product nameAnti-Survivin antibodySee all Survivin primary antibodies ...
  • Description
    Goat polyclonal to Survivin
  • Tested applicationsSandwich ELISA, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide:

    (C)VRRAIEQLAAMD

    , corresponding to C terminal amino acids 131-142 of Human Survivin

  • Positive control
    • Human T cell line MOLT4 lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab27468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit monoclonal [EP2880Y] to Survivin (ab76424). For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab76424 as Detection.
ELISA 1/16000.
WB Use a concentration of 0.01 - 0.5 µg/ml. Detects a band of approximately 18 kDa.

Target

  • FunctionComponent of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
  • Tissue specificityExpressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
  • Sequence similaritiesBelongs to the IAP family.
    Contains 1 BIR repeat.
  • Developmental stageExpression is cell cycle-dependent and peaks at mitosis.
  • DomainThe BIR repeat is necessary and sufficient for HBXIP binding.
  • Post-translational
    modifications
    Ubiquitination is required for centrosomal targeting.
    In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
  • Cellular localizationCytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • API4 antibody
    • API4 antibody
    • Apoptosis Inhibitor 4 antibody
    • Apoptosis Inhibitor 4 antibody
    • Apoptosis inhibitor survivin antibody
    • Apoptosis inhibitor4 antibody
    • Baculoviral IAP repeat containing 5 antibody
    • Baculoviral IAP repeat containing 5 antibody
    • Baculoviral IAP repeat containing protein 5 antibody
    • Baculoviral IAP repeat-containing protein 5 antibody
    • BIRC 5 antibody
    • BIRC 5 antibody
    • BIRC 5 antibody
    • BIRC5 antibody
    • BIRC5_HUMAN antibody
    • EPR 1 antibody
    • EPR 1 antibody
    • EPR 1 antibody
    • IAP4 antibody
    • IAP4 antibody
    • IAP4 antibody
    • Survivin variant 3 alpha antibody
    • SVV antibody
    • SVV antibody
    • SVV antibody
    • TIAP antibody
    • TIAP antibody
    • TIAP antibody
    see all

Anti-Survivin antibody images

Protocols

References for Anti-Survivin antibody (ab27468)

ab27468 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab27468.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"