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- Primary antibodies
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Product name
Anti-Survivin antibody
See all Survivin products (18) ...
Description
Goat polyclonal to Survivin
Tested applications
Sandwich ELISA, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Dog
Immunogen
Synthetic peptide: (C)VRRAIEQLAAMD, corresponding to C terminal amino acids 131-142 of Human Survivin
(C)VRRAIEQLAAMD
Positive control
Human T cell line MOLT4 lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA: Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit monoclonal [EP2880Y] to Survivin (ab76424). For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab76424 as Detection.
ELISA
ELISA: 1/16000.ELISA: 1/16000.
WB: Use a concentration of 0.01 - 0.5 µg/ml. Detects a band of approximately 18 kDa.
Target
Function
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
Tissue specificity
Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
Sequence similarities
Belongs to the IAP family.
Contains 1 BIR repeat.
Developmental stage
Expression is cell cycle-dependent and peaks at mitosis.
Domain
The BIR repeat is necessary and sufficient for HBXIP binding.
Post-translational
modifications
Ubiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
Cellular localization
Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
Target information above from: UniProt accessionO15392
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- API4 antibody
- API4 antibody
- Apoptosis Inhibitor 4 antibody
see all
Database links
- Entrez Gene: 442936 Dog
- Entrez Gene: 332 Human
- Omim: 603352 Human
- SwissProt: Q8I009 Dog
- SwissProt: O15392 Human
- Unigene: 514527 Human
Anti-Survivin antibody images:
Sandwich ELISA - Survivin antibody (ab27468)
Standard curve for Survivin (Analyte: ab87202); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab27468 at 1µg/ml and Detector Antibody abRabbit monoclonal [EP2880Y] to Survivin (ab76424) at 0.5µg/ml. Concentration of Rabbit monoclonal [EP2880Y] to Survivin (ab76424) may vary from lot to lot; please use this curve as guideline.
Western blot - Survivin antibody (ab27468)
Anti-Survivin antibody (ab27468) at 0.01 µg/ml + Molt-4 lysate at 35 µg
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-Survivin antibody (ab27468)
ab27468 has not yet been referenced specifically in any publications.
Publishing research using ab27468? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"