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Immunocytochemistry/ Immunofluorescence - Survivin antibody (ab469)
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Western blot - Survivin antibody (ab469)
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Immunocytochemistry/ Immunofluorescence - Survivin antibody (ab469)
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Product Name
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Survivin antibody
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See all Survivin antibodies (20)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Survivin
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Immunogen
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Recombinant full length protein (Human).
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Reacts with
(species key)
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Hu, Ms, Rat, Cat, Cow, Dog
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Specificity
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Specific for survivin.
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Tested applications
(see key)
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ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/250 (see Abreview by William Moore; Fix with formaldehyde).
IHC-P: Use at a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IP: Use 5-7 µg
WB: Use at a concentration of 1 µg/ml. Found to work at 1/5000 dilution (see PMID: 16847456). Predicted molecular weight: 16 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: HeLa cell lysate
ICC: HeLa cells
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
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Relevance
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Survivin is an anti-apoptotic protein, expressed in the G2/M cell cycle phase. At the onset of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptotic function and increased caspase 3 activity.
Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein. Survivin might affect regulation of neural cell proliferative responses after brain injury. Survivin is also an inhibitor of apoptosis (IAP), or programmed cell death, that is selectively overexpressed in common human cancers.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS with 0.05% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.970 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Survivin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Survivin antibody images, references, products related to ab469 and other tools.
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Survivin antibody images:
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 Immunocytochemistry/ Immunofluorescence - Survivin antibody (ab469)
HeLa cells in prometaphase, metaphase and anaphase stained with anti-Survivin (green), anti-tubulin (red) and DAPI (blue). These images were kindly supplied as part of the review submitted by William Moore, University of Dundee, UK. William Moore, Org unknown, Country unknown
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Western blot - Survivin antibody (ab469)
All lanes : Survivin antibody (ab469) at 1 µg/ml
Lane 1 : HeLa Nuclear
Lane 2 : HeLa whole cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor anti-rabbit at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 16 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa,50 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - Survivin antibody (ab469)
ab469 at a 1/400 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then was detected using a Texas Red conjugated Goat anti-rabbit antibody. This image is courtesy of an Abreview by Sandrine Ruchaud submitted on 30 March 2006.
See Abreview
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Immunoprecipitation - Survivin antibody (ab469)
ab469 immunoprecipitating Survivin from HeLa cell lysate. HeLa cells were lysed after colcemid block (ON) and immunoprecipitated with ab469 at a 1/1000 dilution. HeLa cells clear lysate (Lane 1) as well as the bound material (Lane 2) were loaded on a 15 % acrylamide gel. An HRP conjugated Donkey Anti-rabbit IgG was used as the secondary antibody. This image is courtesy of an Abreview by Sandrine Ruchaud submitted on 12 April 2006.
See Abreview
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Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Survivin antibody (ab469)
ab469 staining Survivin from Ovarian carcionoma tumour tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed (Citrate buffer pH=6, microwave oven) and the tissue was then formaldehyde fixed and blocked (Hydrogen peroxide 0.03%). An HRP conjugated goat anti-rabbit was used as the secondary antibody. This image is courtesy of an Abreview submitted by Dr Ben Davidson
See Abreview
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References for Survivin antibody (ab469)
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This product has been used in: (two most recent references)
Dallaglio K et al. Endogenous survivin modulates survival and proliferation in UVB-treated human keratinocytes. Exp Dermatol 18:464-71 (2009). WB; Human. PubMed: 19320741
Ferri F et al. Non-coding murine centromeric transcripts associate with and potentiate Aurora B kinase. Nucleic Acids Res 37:5071-80 (2009). ChIP; Mouse. PubMed: 19542185
See all 32 publication references for this product.
If you publish research using ab469 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Survivin
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Survivin antibody - more information
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Customer reviews (feedback) regarding Survivin antibody |
Customer FAQs regarding Survivin antibody |
Protocols for Survivin antibody |
Price and availability of products related to Survivin antibody |
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Survivin antibody - related products:
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Survivin antibody - other tools:
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Contact Abcam with a Technical Enquiry about Survivin antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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