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Products:Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Overview
Product name
Anti-Survivin antibody
See all Survivin products (18) ...
Description
Rabbit polyclonal to Survivin
Tested applications
WB, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Cow, Cat, Dog, Pig
Immunogen
Synthetic peptide derived from within residues 100 to the C-terminus of Human Survivin.
.
Positive control
HL60 Whole Cell Lysate and Human Fetal Kidney Tissue Lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 16 kDa.Can be blocked with Survivin peptide (ab13761).
ICC/IF: Use a concentration of 1 µg/ml
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Function
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
Tissue specificity
Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
Sequence similarities
Belongs to the IAP family.
Contains 1 BIR repeat.
Developmental stage
Expression is cell cycle-dependent and peaks at mitosis.
Domain
The BIR repeat is necessary and sufficient for HBXIP binding.
Post-translational
modifications
Ubiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
Cellular localization
Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
Target information above from: UniProt accessionO15392
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- API4 antibody
- Apoptosis Inhibitor 4 antibody
- Apoptosis inhibitor survivin antibody
see all
Database links
- Entrez Gene: 493835 Cat
- Entrez Gene: 414925 Cow
- Entrez Gene: 442936 Dog
- Entrez Gene: 332 Human
- Entrez Gene: 397266 Pig
- Omim: 603352 Human
- SwissProt: Q6I6F4 Cat
- SwissProt: Q6J1J1 Cow
see all
Anti-Survivin antibody images:
Immunocytochemistry/ Immunofluorescence - Survivin antibody (ab8228)
ICC/IF image of ab8228 stained SH-SY-5Y cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab8228, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Survivin antibody (ab8228)
ab8228 staining Survivin in human breast cancer tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/250 (or 1/1500) and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Survivin antibody (ab8228)
ab8228 staining Survivin in human stomach cancer tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in EDTA for 30 minutes at 1000C. The primary antibody was diluted 1/200 and incubated with sample for 30 minutes at 25°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.
This image is a courtesy of Anonymous Abreview
Immunocytochemistry/ Immunofluorescence - Survivin antibody (ab8228)
IHC image of Survivin staining in human lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8228, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - Survivin antibody (ab8228)
All lanes : Anti-Survivin antibody (ab8228) at 2 µg/ml
Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 2 : Kidney (Human) Tissue Lysate - fetal normal tissue (ab30204)
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 16,18 kDa (why is the actual band size different from the predicted?)
Exposure time : 8 minutes
This antibody was blocked using 3% milk. Using a higher concentration of blocking buffer may help to further reduce non-specific bands.
References for Anti-Survivin antibody (ab8228)
This product has been referenced in:
- Yang Let al. Biological Function and Prognostic Significance of Peroxisome Proliferator-Activated Receptor {delta} in Rectal Cancer. Clin Cancer Res 17:3760-70 (2011). WB; Human.Read more (PubMed: 21531809) »
- Green SRet al. Combination of sapacitabine and HDAC inhibitors stimulates cell death in AML and other tumour types. Br J Cancer 103:1391-9 (2010). WB; Human.Read more (PubMed: 20924380) »
See all 2 publications for this product
Publishing research using ab8228? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"