RabMAb

Anti-SynGAP antibody [EPR2883Y] (ab77235)

Overview

  • Product nameAnti-SynGAP antibody [EPR2883Y]
    See all SynGAP primary antibodies
  • Description
    Rabbit monoclonal [EPR2883Y] to SynGAP
  • Tested applicationsWB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues on the C terminus of human SynGAP

  • Positive control
    • Fetal brain lysate
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab77235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 147 kDa.
Flow Cyt 1/50.
ICC/IF Use at an assay dependent dilution.
  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • FunctionMajor constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR-mediated miniature excitatory postsynaptic currents. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits.
    • Involvement in diseaseDefects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5) [MIM:612621]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRD5 patients show global developmental delay with delayed motor development, hypotonia, moderate-to-severe mental retardation, and severe language impairment. Autism can be present in some patients.
    • Sequence similaritiesContains 1 C2 domain.
      Contains 1 PH domain.
      Contains 1 Ras-GAP domain.
    • Post-translational
      modifications
      Phosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp761G1421 antibody
      • KIAA1938 antibody
      • MRD5 antibody
      • Neuronal RasGAP antibody
      • OTTHUMP00000064825 antibody
      • p135 SynGAP antibody
      • Ras GTPase activating protein SynGAP antibody
      • Ras GTPase-activating protein SynGAP antibody
      • RASA 1 antibody
      • RASA 5 antibody
      • RASA1 antibody
      • RASA5 antibody
      • SYGP1_HUMAN antibody
      • Synaptic Ras GAP 1 antibody
      • Synaptic Ras GTPase activating protein 1 antibody
      • Synaptic Ras GTPase activating protein 1 homolog antibody
      • Synaptic Ras GTPase activating protein 135kDa antibody
      • Synaptic Ras GTPase activating protein antibody
      • Synaptic Ras GTPase-activating protein 1 antibody
      • Synaptic Ras-GAP 1 antibody
      • SYNGAP 1 antibody
      • SYNGAP1 antibody
      see all

    Anti-SynGAP antibody [EPR2883Y] images

    • Anti-SynGAP antibody [EPR2883Y] (ab77235) at 1/1000 dilution + fetal brain lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 147 kDa
      Observed band size : 147 kDa
      Additional bands at : 50 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-SynGAP antibody [EPR2883Y] (ab77235)

    ab77235 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you very much for your interest in ab77235.

    Unfortunately, we couldn't BLAST the immunogen sequence against zebrafish since we couldn't find the zebrafish SYNGAP1 protein sequence online. If you have it, we can BLAST it agains the immu...

    Read More

    We tested rat brain and did not observe a band at the predicted MW so we do not recommend the product for IP.

    Yes, the clone ID's are the same. Millipore sells the same SynGAP antibody.
    I hope this is helpful. Please contact us again if you have any further questions.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"