Anti-SynGAP (phospho S1123) antibody (ab16649)

Overview

  • Product nameAnti-SynGAP (phospho S1123) antibodySee all SynGAP primary antibodies ...
  • Description
    Rabbit polyclonal to SynGAP (phospho S1123)
  • Tested applicationsWB, IHC (PFA fixed), ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Human

    Does not react with

    Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1100 - 1200 of Rat SynGAP, phosphorylated at S1123.

    (Peptide available as ab16982.)

  • Positive control
    • SynGAP is enriched in the hippocampus, rat hippocampus can be used as a control for SynGAP (phospo S1123) expression. A predicted positive control would be ischaemic rat brain tissue based on publication by Song et al, 2004 (see references section).

Properties

Applications

Our Abpromise guarantee covers the use of ab16649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Can be blocked with Rat SynGAP (phospho S1123) peptide (ab16982).
IHC (PFA fixed) Use a concentration of 0.2 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionMajor constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR-mediated miniature excitatory postsynaptic currents. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits.
  • Involvement in diseaseDefects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5) [MIM:612621]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRD5 patients show global developmental delay with delayed motor development, hypotonia, moderate-to-severe mental retardation, and severe language impairment. Autism can be present in some patients.
  • Sequence similaritiesContains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 Ras-GAP domain.
  • Post-translational
    modifications
    Phosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp761G1421 antibody
    • KIAA1938 antibody
    • MRD5 antibody
    • Neuronal RasGAP antibody
    • OTTHUMP00000064825 antibody
    • p135 SynGAP antibody
    • Ras GTPase activating protein SynGAP antibody
    • Ras GTPase-activating protein SynGAP antibody
    • RASA 1 antibody
    • RASA 5 antibody
    • RASA1 antibody
    • RASA5 antibody
    • SYGP1_HUMAN antibody
    • Synaptic Ras GAP 1 antibody
    • Synaptic Ras GTPase activating protein 1 antibody
    • Synaptic Ras GTPase activating protein 1 homolog antibody
    • Synaptic Ras GTPase activating protein 135kDa antibody
    • synaptic Ras GTPase activating protein antibody
    • Synaptic Ras GTPase-activating protein 1 antibody
    • Synaptic Ras-GAP 1 antibody
    • SYNGAP 1 antibody
    • SYNGAP1 antibody
    see all

Anti-SynGAP (phospho S1123) antibody images

  • Immuofluorescent staining for SynGap phospho S1123 in the hippocampus of a naïve rat using antibody ab16649. [A] Immunostaining was observed in the cytoplasm of a few hippocampal neurons (Picture taken with a X10 objective). Higher power magnification of the same hippocampal section is shown in image B (X 40 objective). Tissue preparation: rat brain tissue was perfusion fixed (4% PFA) followed by post fix and cryoprotection in 20% sucrose before freezing in OCT. 30µm coronal sections were cut on a cryostat for free floating IHC. Primary antibody ab16649 was used at 1/3000 (0.2µg/ml) incubated overnight at room temperature. Secondary antibody used: anti-rabbit Alexa fluor 488 (1/1000) incubated for 2 hours at room temperature.

  • ICC/IF image of ab16649 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16649, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-SynGAP (phospho S1123) antibody (ab16649)

This product has been referenced in:
  • Huo XL  et al. Efficacy of lovastatin on learning and memory deficits caused by chronic intermittent hypoxia-hypercapnia: through regulation of NR2B-containing NMDA receptor-ERK pathway. PLoS One 9:e94278 (2014). WB ; Rat . Read more (PubMed: 24718106) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 5 µg
Gel Running Conditions Non-reduced Denaturing
Sample Rat Tissue lysate - whole (hippocampus tissue lysate)
Specification hippocampus tissue lysate
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Jul 07 2014

Thank you for contacting us. The immunogen sequence is ITKQHS"p"QTPST where the phosphorylation is at the serine with the "p" after it. The numbering on the datasheet refers to isoform 2 of rat SynGAP, as described at this UniProt page: http://ww...

Read More
Application Western blot
Sample Rat Tissue lysate - whole (Rat brain tissue homogenate)
Loading amount 30 µg
Specification Rat brain tissue homogenate
Gel Running Conditions Non-reduced Denaturing (8%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Johanna Schumann

Verified customer

Submitted Mar 17 2010

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Sections of rat brain)
Specification Sections of rat brain
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

Submitted May 18 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"