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Anti-Synaptobrevin antibody [SP11] (ab11104)

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Overview

Product name

Anti-Synaptobrevin antibody [SP11]
See all Synaptobrevin products (6) ...

Description

Mouse monoclonal [SP11] to Synaptobrevin

Specificity

ab11104 recognises the human synaptic vesicle protein synaptobrevin, VAMP; it is not known whether it recognises VAMP1 or VAMP2. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

Tested applications

WB, IHC-P, ELISAmore details

Cross reactivity

Reacts with

Human, Pig

Immunogen

Crude human synaptic immunoprecipitate.

Positive control

human brain lysate

General notes

This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

Purified IgG prepared by affinity chromatography from tissue culture supernatant.

Clonality

Monoclonal

Clone number

SP11

Myeloma

NS0

Isotype

IgG1

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Neuroscience >> Neurotransmission >> Secretory Vesicles >> SNAPs & SNAREs

  • Western blot - Synaptobrevin antibody [SP11] (ab11104)Western blot - Synaptobrevin antibody [SP11] (ab11104) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab11104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Synaptobrevins are highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Synaptobrevins, Syntaxins, and the 25 kDa synaptosomal associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane.

Cellular localization

Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane. Single-pass type IV membrane protein. Cell junction > synapse > synaptosome. Isoform 3 is found in mitochondria, mitochondrial outer membrane.

Alternative names

  • SYB antibody
  • SYB1 antibody
  • Synaptobrevin 1 antibody
  • VAMP antibody
  • VAMP1 antibody
  • Vesicle associated membrane protein 1 antibody
  • Vesicle associated membrane protein antibody
see all

Database links

Anti-Synaptobrevin antibody [SP11] images:

  Western blot - Synaptobrevin antibody [SP11] (ab11104)

Western blot - Synaptobrevin antibody [SP11] (ab11104)

Anti-Synaptobrevin antibody [SP11] (ab11104) + Human brain extract

Secondary
F(ab')2 Rabbit anti mouse IgG:HRP

Predicted band size : 13 kDa
Observed band size : 14 kDa (why is the actual band size different from the predicted?)

References for Anti-Synaptobrevin antibody [SP11] (ab11104)

ab11104 has not yet been referenced specifically in any publications.

Publishing research using ab11104? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"