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Anti-Synaptogyrin 4 antibody (ab27480) at 0.01 µg/ml + Human brain lysate (35µg protein in RIPA buffer).
Predicted band size : 26 kDa
Observed band size : 23 kDa (why is the actual band size different from the predicted?)
Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour. Detected by chemiluminescence. A minor band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunising peptide.
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