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Products:Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Overview
Product name
Anti-Synaptogyrin 4 antibody
See all Synaptogyrin 4 products (3) ...
Description
Goat polyclonal to Synaptogyrin 4
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: HIPKSLQELANSC, corresponding to amino acids 2-14 of Human Synaptogyrin 4
HIPKSLQELA NSC
Positive control
Human brain lysate for Western blot.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
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Western blot - Synaptogyrin 4 antibody (ab27480)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
Application notes
ELISA: 1/128000.
WB: Use at a concentration of 0.01 - 01 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 26 kDa). A minor band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunising peptide. Can be blocked with Synaptogyrin 4 peptide (2-14) (ab45658).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Sequence similarities
Belongs to the synaptogyrin family.
Contains 1 MARVEL domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionO95473
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MGC125805 antibody
- SNG4_HUMAN antibody
- Synaptogyrin-4 antibody
- SYNGR4 antibody
Database links
- Entrez Gene: 23546 Human
- Omim: 608373 Human
- SwissProt: O95473 Human
- Unigene: 408333 Human
Anti-Synaptogyrin 4 antibody images:
Western blot - Synaptogyrin 4 antibody (ab27480)
Anti-Synaptogyrin 4 antibody (ab27480) at 0.01 µg/ml + Human brain lysate (35µg protein in RIPA buffer).
Predicted band size : 26 kDa
Observed band size : 23 kDa (why is the actual band size different from the predicted?)
Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour. Detected by chemiluminescence. A minor band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunising peptide.
References for Anti-Synaptogyrin 4 antibody (ab27480)
ab27480 has not yet been referenced specifically in any publications.
Publishing research using ab27480? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"