Anti-Synaptojanin antibody [AC1] (ab24591)
Key features and details
- Mouse monoclonal [AC1] to Synaptojanin
- Suitable for: ICC/IF
- Reacts with: Rat
- Isotype: unknown
Overview
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Product name
Anti-Synaptojanin antibody [AC1]
See all Synaptojanin primary antibodies -
Description
Mouse monoclonal [AC1] to Synaptojanin -
Host species
Mouse -
Specificity
ab24591 is specific for rat synsptojanin 1 protein. It may cross-react only very weakly with human synaptojanin 1. ab24591 does not cross react with mouse. -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Recombinant fragment, corresponding to C terminal amino acids 1156-1286 of Rat Synaptojanin
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General notes
The C-terminal proline-rich region of Synaptojanin 1 mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 and GRB2.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
The C-terminal proline-rich region of Synaptojanin 1 mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 and GRB2. -
Clonality
Monoclonal -
Clone number
AC1 -
Isotype
unknown -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (1) |
1/20 - 1/100.
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Notes |
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ICC/IF
1/20 - 1/100. |
Target
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Function
Inositol 5-phosphatase which has a role in clathrin-mediated endocytosis. -
Tissue specificity
Concentrated at clathrin-coated endocytic intermediates in nerve terminals. Isoform 1 is more enriched than isoform 2 in developing brain as well as non-neuronal cells. Isoform 2 is very abundant in nerve terminals. -
Sequence similarities
Belongs to the synaptojanin family.
In the central section; belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SAC domain. -
Domain
Binds to EPS15 (a clathrin coat-associated protein) via a C-terminal domain containing three Asn-Pro-Phe (NPF) repeats.
The C-terminal proline-rich region mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 and GRB2. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 85238 Rat
- SwissProt: Q62910 Rat
- Unigene: 22685 Rat
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Alternative names
- 4 antibody
- 5-trisphosphate 5-phosphatase 1 antibody
- Inositol 5' phosphatase (synaptojanin 1) antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab24591 has been referenced in 5 publications.
- Latimer CS et al. Vitamin D prevents cognitive decline and enhances hippocampal synaptic function in aging rats. Proc Natl Acad Sci U S A 111:E4359-66 (2014). Rat . PubMed: 25267625
- Nemoto Y et al. Identification and characterization of a synaptojanin 2 splice isoform predominantly expressed in nerve terminals. J Biol Chem 276:41133-42 (2001). PubMed: 11498538
- Haffner C et al. Synaptojanin 1: localization on coated endocytic intermediates in nerve terminals and interaction of its 170 kDa isoform with Eps15. FEBS Lett 419:175-80 (1997). PubMed: 9428629
- Ringstad N et al. The SH3p4/Sh3p8/SH3p13 protein family: binding partners for synaptojanin and dynamin via a Grb2-like Src homology 3 domain. Proc Natl Acad Sci U S A 94:8569-74 (1997). PubMed: 9238017
- Nemoto Y et al. Synaptojanin 2, a novel synaptojanin isoform with a distinct targeting domain and expression pattern. J Biol Chem 272:30817-21 (1997). PubMed: 9388224