Anti-Synaptophysin antibody [SP17] (ab24729)

Overview

  • Product nameAnti-Synaptophysin antibody [SP17]
    See all Synaptophysin primary antibodies
  • Description
    Mouse monoclonal [SP17] to Synaptophysin
  • SpecificitySP17 reacts with native, denatured and recombinant synaptic vesicle protein synaptophysin expressed in CHO cells.
  • Tested applicationsWB, ICC, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human, Pig
  • Immunogen

    Tissue/ cell preparation of a crude synaptic preparation from the post mortem brain (Human)

  • General notes


    The antibody is particularly valueable for immunocytochemical studies of the brain due to its stability in formalin sections.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: Ascites
  • Concentration information loading...
  • PurityAscites
  • Primary antibody notes The antibody is particularly valueable for immunocytochemical studies of the brain due to its stability in formalin sections.
  • Clonality Monoclonal
  • Clone numberSP17
  • IsotypeIgM
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab24729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 40 kDa.
ICC 1/1000.
ELISA 1/5000.

Target

  • FunctionPossibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
  • Tissue specificityCharacteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
  • Involvement in diseaseDefects in SYP are the cause of mental retardation X-linked SYP-related (MRXSYP) [MIM:300802]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period.
  • Sequence similaritiesBelongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.
  • DomainThe calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
  • Post-translational
    modifications
    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Major synaptic vesicle protein P38 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • SYP antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

References for Anti-Synaptophysin antibody [SP17] (ab24729)

ab24729 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Synaptophysin antibodies ab11105 and ab24729 are derived from two different monoclonal clones: [SP15] and [SP17]. They were raised against different immunogens and recognise different epitopes. Both antisera have als...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"