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Anti-Synaptophysin antibody [SP17] (ab24729)

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Overview

  • Product nameAnti-Synaptophysin antibody [SP17]See all Synaptophysin primary antibodies ...
  • Description
    Mouse monoclonal [SP17] to Synaptophysin
  • SpecificitySP17 reacts with native, denatured and recombinant synaptic vesicle protein synaptophysin expressed in CHO cells.
  • Tested applicationsWB, ICC, ELISA more details
  • Species reactivity
    Reacts with: Rat, Human, Pig
  • Immunogen

    Tissue / cell preparation of a crude synaptic preparation from the post mortem brain (Human)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: Ascites
  • Concentration information loading...
  • PurityAscites
  • Primary antibody notes The antibody is particularly valueable for immunocytochemical studies of the brain due to its stability in formalin sections.
  • Clonality Monoclonal
  • Clone numberSP17
  • IsotypeIgM
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab24729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000. Predicted molecular weight: 40 kDa.
ICC ICC: 1/1000.
ELISA ELISA: 1/5000.

Target

  • FunctionPossibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
  • Tissue specificityCharacteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
  • Involvement in diseaseDefects in SYP are the cause of mental retardation X-linked SYP-related (MRXSYP) [MIM:300802]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period.
  • Sequence similaritiesBelongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.
  • DomainThe calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
  • Post-translational
    modifications    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.

    • Target information above from: UniProt accession P08247 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Major synaptic vesicle protein P38 antibodyMRXSYP antibodySyn p38 antibody
        Synaptophysin antibodySYP antibodySYPH antibodySYPH_HUMAN antibodySypI antibody
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    References for Anti-Synaptophysin antibody [SP17] (ab24729)

    ab24729 has not yet been referenced specifically in any publications.

    Publishing research using ab24729 ? Please let us know so that we can cite the reference in this datasheet

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    I'm interesting in Synaptophysin Ab[SP15] or [SP17], what a difference between them and also, I'd like to know if they were tested on the frozen tissue too.

    Thank you for your enquiry. Synaptophysin antibodies ab11105 and ab24729 are derived from two different monoclonal clones: [SP15] and [SP17]. They were raised against different immunogens and recognise different epitopes. Both antisera have also b...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"