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Anti-Synaptophysin antibody [SY38] (ab8049)

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Overview

Product name

Anti-Synaptophysin antibody [SY38]
See all Synaptophysin products (24) ...

Description

Mouse monoclonal [SY38] to Synaptophysin

Specificity

The antibody is specific for Synaptophysin. The specificity was ascertained by immunoblotting and immunohistochemistry.

Tested applications

WB, Flow Cyt, ICC/IF, IHC-Fr, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Hamster, Cow, Human

Immunogen

Presynaptic vesicles.

Positive control

Adrenal pheochromocytoma and pancreatic islet cell neoplasm

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Ascitic fluid with 0.1% sodium azide

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

SY38

Isotype

IgG1

Research areas

Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Neuroscience >> Neurotransmission >> Secretory Vesicles >> SNAPs & SNAREs

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Synaptophysin antibody [SY38] (ab8049)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Synaptophysin antibody [SY38] (ab8049) image (enlarge)

  • Western blot - Synaptophysin antibody [SY38] (ab8049)Western blot - Synaptophysin antibody [SY38] (ab8049) image (enlarge)

  • Flow Cytometry-Synaptophysin antibody [SY38](ab8049)Flow Cytometry-Synaptophysin antibody [SY38](ab8049) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab8049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.

Tissue specificity

Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.

Involvement in disease

Defects in SYP are the cause of mental retardation X-linked SYP-related (MRXSYP) [MIM:300802]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period.

Sequence similarities

Belongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain.

Domain

The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.

Post-translational
modifications

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

Cellular localization

Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.

Target information above from: UniProt accessionP08247 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Major synaptic vesicle protein P38 antibody
  • Syn p38 antibody
  • Synaptophysin antibody
  • SYP antibody
  • SYPH antibody
  • SYPH_HUMAN antibody
  • SypI antibody
see all

Database links

see all

Anti-Synaptophysin antibody [SY38] images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Synaptophysin antibody [SY38] (ab8049)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Synaptophysin antibody [SY38] (ab8049)

The pictures show immunohistochemical staining of normal mucosa and neuroendocrine tumour of the small intestine.  The antibody (ab8049) stains positive in a 1:10 dilution in nerve cells of the colon as well as some cells in normal colon mucosa and some neuroendocrine tumors of the digestive tract. Staining protocol: Mouse monoclonal to Synaptophysin (ab8049), diluted 1:10 in PBS plus BSA, 60 min at room temperature,  
secondary antibody: HRP solution with anti mouse polyclonal antibodies, 30min. DAB staining 10 min; Hematoxylin 30 sec.

These images were kindly supplied as part of the review submitted by Karin Birkenkamp-Demtroeder.

  Western blot - Synaptophysin antibody [SY38] (ab8049)

Western blot - Synaptophysin antibody [SY38] (ab8049)

All lanes : Anti-Synaptophysin antibody [SY38] (ab8049) at 1/500 dilution

Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed at 1/3000 dilution

Predicted band size : 34 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)

  Flow Cytometry-Synaptophysin antibody [SY38](ab8049)

Flow Cytometry-Synaptophysin antibody [SY38](ab8049)

Overlay histogram showing PC12 cells stained with ab8049 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8049, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

References for Anti-Synaptophysin antibody [SY38] (ab8049)

This product has been referenced in:

  • Devi L & Ohno M Mitochondrial dysfunction and accumulation of the ß-secretase-cleaved C-terminal fragment of APP in Alzheimer's disease transgenic mice. Neurobiol Dis : (2011). WB; Mouse.Read more (PubMed: 21933711) »
  • Scavone JL  et al. Characterization of cannabinoid-1 receptors in the locus coeruleus: relationship with mu-opioid receptors. Brain Res 1312:18-31 (2010). IHC (PFA fixed); Rat.Read more (PubMed: 19931229) »

See all 15 publications for this product

Publishing research using ab8049? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"