Overview

  • Product nameAnti-Synaptophysin antibodySee all Synaptophysin primary antibodies ...
  • Description
    Rabbit polyclonal to Synaptophysin
  • Tested applicationsWB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide from rat or mouse Synaptophysin conjugated to an immunogenic carrier protein.

  • Positive control
    • 1 day- old mouse cortical cells, rat trigeminal nerve tissue, rat brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab68851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
IHC-Fr
ICC/IF
  • Application notesICC/IF: 1/2000.
    IHC-P: 1/300 - 1/3000. Perform antigen retrieval before commencing with IHC staining protocol.
    IHC-Fr: 1/300 - 1/3000.
    WB: 1/300 - 1/3000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPossibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
    • Tissue specificityCharacteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
    • Involvement in diseaseDefects in SYP are the cause of mental retardation X-linked SYP-related (MRXSYP) [MIM:300802]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period.
    • Sequence similaritiesBelongs to the synaptophysin/synaptobrevin family.
      Contains 1 MARVEL domain.
    • DomainThe calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
    • Post-translational
      modifications
      Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
    • Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • Major synaptic vesicle protein P38 antibody
      • MRXSYP antibody
      • Syn p38 antibody
      • Synaptophysin antibody
      • SYP antibody
      • SYPH antibody
      • SYPH_HUMAN antibody
      • SypI antibody
      see all

    Anti-Synaptophysin antibody images

    • All lanes : Anti-Synaptophysin antibody (ab68851) at 1/1000 dilution

      Lane 1 : Rat brain lysate, untreated
      Lane 2 : Rat brain lysate, cells pretreated with the immunising peptide


      Predicted band size : 33 kDa
      Observed band size : 33 kDa
      Additional bands at : >75 kDa. We are unsure as to the identity of these extra bands.
    • 1 day old mouse cortical cell culture stained with ab68851 at 1/2000 dilution. DAPI counter stained.
    • Rat trigeminal nerve tissue stained with ab68851 at 1/2000 dilution.

    References for Anti-Synaptophysin antibody (ab68851)

    This product has been referenced in:
    • Micheva KD  et al. Single-synapse analysis of a diverse synapse population: proteomic imaging methods and markers. Neuron 68:639-53 (2010). Mouse . Read more (PubMed: 21092855) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab68851.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"