Anti-Synaptophysin antibody (ab68851)

Overview

• Product nameAnti-Synaptophysin antibodySee all Synaptophysin primary antibodies ...
• Description
  Rabbit polyclonal to Synaptophysin
• Tested applicationsWB, IHC-P, IHC-Fr, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat
  
• Immunogen

  Synthetic peptide from rat or mouse Synaptophysin conjugated to an immunogenic carrier protein.

• Positive control1 day- old mouse cortical cells, rat trigeminal nerve tissue, rat brain lysate.

Properties

• FormLyophilised:Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
• Storage instructionsStore at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Concentration information loading...
• PurityWhole antiserum
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Neuroscience
  • Neurotransmission
  • Secretory Vesicles
  • SNAPs & SNAREs

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Synapse marker

Applications

Target

• FunctionPossibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
• Tissue specificityCharacteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
• Involvement in diseaseDefects in SYP are the cause of mental retardation X-linked SYP-related (MRXSYP) [MIM:300802]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period.
• Sequence similaritiesBelongs to the synaptophysin/synaptobrevin family.
  Contains 1 MARVEL domain.
• DomainThe calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
• Post-translational
  modificationsUbiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
• Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.

Target information above from: UniProt accession P08247 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 20977 Mouse
  • Entrez Gene: 24804 Rat
  • SwissProt: Q62277 Mouse
  • SwissProt: P07825 Rat
  • Unigene: 223674 Mouse
  • Unigene: 11067 Rat

• Alternative names
  Major synaptic vesicle protein P38 antibodyMRXSYP antibodySyn p38 antibody

  Synaptophysin antibodySYP antibodySYPH antibodySYPH_HUMAN antibodySypI antibody

  see all

Anti-Synaptophysin antibody images

• 

  Western blot - Synaptophysin antibody (ab68851)
  All lanes : Anti-Synaptophysin antibody (ab68851) at 1/1000 dilution
  
  Lane 1 : Rat brain lysate, untreated
  Lane 2 : Rat brain lysate, cells pretreated with the immunising peptide
  
  
  Predicted band size : 33 kDa
  Observed band size : 33 kDa
  Additional bands at : >75 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunocytochemistry/ Immunofluorescence - Synaptophysin antibody (ab68851)
  1 day old mouse cortical cell culture stained with ab68851 at 1/2000 dilution. DAPI counter stained.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Synaptophysin antibody (ab68851)
  Rat trigeminal nerve tissue stained with ab68851 at 1/2000 dilution.