Anti-Synaptophysin antibody (ab7837)
Key features and details
- Rabbit polyclonal to Synaptophysin
- Suitable for: ICC/IF
- Reacts with: Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Synaptophysin antibody
See all Synaptophysin primary antibodies -
Description
Rabbit polyclonal to Synaptophysin -
Host species
Rabbit -
Specificity
This antibody reacts with a hydrophilic peptide sequence of synaptophysin. -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: PC12 Cells
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 µg/ml.
|
Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. -
Tissue specificity
Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. -
Involvement in disease
Mental retardation, X-linked, SYP-related -
Sequence similarities
Belongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain. -
Domain
The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein. -
Post-translational
modificationsUbiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
Cellular localization
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome. - Information by UniProt
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Database links
- Entrez Gene: 24804 Rat
- SwissProt: P07825 Rat
- Unigene: 11067 Rat
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Alternative names
- Major synaptic vesicle protein p38 antibody
- MRX96 antibody
- MRXSYP antibody
see all
Images
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ICC/IF image of ab7837 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7837, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab7837 has been referenced in 11 publications.
- Kourgiantaki A et al. Neural stem cell delivery via porous collagen scaffolds promotes neuronal differentiation and locomotion recovery in spinal cord injury. NPJ Regen Med 5:12 (2020). PubMed: 32566251
- Zeineldin M et al. MYCN amplification and ATRX mutations are incompatible in neuroblastoma. Nat Commun 11:913 (2020). PubMed: 32060267
- Gan Q et al. Effects of Amylin Against Amyloid-ß-Induced Tauopathy and Synapse Loss in Primary Neurons. J Alzheimers Dis 70:1025-1040 (2019). PubMed: 31306122
- Pathak NM et al. Novel dual incretin agonist peptide with antidiabetic and neuroprotective potential. Biochem Pharmacol 155:264-274 (2018). PubMed: 30028989
- Denver P et al. Inflammation, insulin signaling and cognitive function in aged APP/PS1 mice. Brain Behav Immun 70:423-434 (2018). PubMed: 29604345