Anti-Synaptotagmin 1 antibody (ab131551)

Overview

• Product nameAnti-Synaptotagmin 1 antibodySee all Synaptotagmin 1 primary antibodies ...
• Description
  Rabbit polyclonal to Synaptotagmin 1
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide conjugated to KLH, corresponding to a region around amino acids 307-311 (GLSDP) of Human Synaptotagmin 1 (NP_001129277.1).

  Run BLAST with Run BLAST with
• Positive controlHeLa cells; Mouse brain and Rat brain tissue extracts

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
  Note: PBS without Mg²⁺ and Ca²⁺
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Trafficking
  • Vesicle Transport
  • Regulation

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Synapse marker

  • Neuroscience
  • Neurotransmission
  • Secretory Vesicles
  • Other

  • Neuroscience
  • Neurotransmission
  • Secretory Vesicles
  • Regulation

Applications

Target

• FunctionMay have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
• Sequence similaritiesBelongs to the synaptotagmin family.
  Contains 2 C2 domains.
• DomainThe first C2 domain mediates Ca(2+)-dependent phospholipid binding.
  The second C2 domain mediates interaction with SV2A and STN2.
• Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. Cytoplasm. Synaptic vesicles and chromaffin granules.

Target information above from: UniProt accession P21579 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6857 Human
  • Entrez Gene: 20979 Mouse
  • Entrez Gene: 25716 Rat
  • Omim: 185605 Human
  • SwissProt: P21579 Human
  • SwissProt: P46096 Mouse
  • SwissProt: P21707 Rat
  • Unigene: 310545 Human

  • Unigene: 289702 Mouse
  • Unigene: 216272 Rat

  see all

• Alternative names
  DKFZp781D2042 antibodyp65 antibodySVP65 antibody

  Synaptotagmin I antibodySynaptotagmin-1 antibodySYT antibodySyt1 antibodySYT1_HUMAN antibodySytI antibody

  see all

Anti-Synaptotagmin 1 antibody images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Synaptotagmin 1 antibody (ab131551)
  Immunofluorescent analysis of methanol-fixed HeLa cells labelling Synaptotagmin 1 with ab131551 at 1/100 dilution.
• 

  Western blot - Anti-Synaptotagmin 1 antibody (ab131551)
  All lanes : Anti-Synaptotagmin 1 antibody (ab131551) at 1/500 dilution
  
  Lane 1 : Mouse brain tissue extract
  Lane 2 : Rat brain tissue extract
  
  
  Predicted band size : 47 kDa