![Western blot - Synaptotagmin antibody [ASV30] (ab13259) image](#Synaptotagmin-Primary-antibodies-ab13259-1.jpg)
 image](#Synaptotagmin-Primary-antibodies-ab13259-2.jpg)
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Product name
Anti-Synaptotagmin antibody [ASV30]
See all Synaptotagmin products (6) ...
Description
Mouse monoclonal [ASV30] to Synaptotagmin
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
Rat brain synaptic junctional protein complexes.
Positive control
Mouse or Rat brain tissue extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody has been affinity purified.
Clonality
Monoclonal
Clone number
ASV30
Isotype
IgG2a
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Other
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Munc18
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2 µg/ml.
ICC/IF: Use a concentration of 5 µg/ml.
Target
Relevance
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first C2 domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
Cellular localization
Cytoplasmic
Alternative names
- DKFZp781D2042 antibody
- P65 antibody
- P65 antibody
see all
Database links
- Entrez Gene: 20979 Mouse
- Entrez Gene: 25716 Rat
- SwissProt: P46096 Mouse
- SwissProt: P21707 Rat
- Unigene: 216272 Rat
Anti-Synaptotagmin antibody [ASV30] images:
Western blot - Synaptotagmin antibody [ASV30] (ab13259)
![Western blot - Synaptotagmin antibody [ASV30] (ab13259)](/ps/datasheet/images/13/ab13259/Synaptotagmin-Primary-antibodies-ab13259-1.jpg)
All lanes : Anti-Synaptotagmin antibody [ASV30] (ab13259) at 1/1000 dilution
Lane 1 : Molecular weight ladder
Lane 2 : Lysates prepared from mouse brain
Lane 3 : Lysates prepared from rat brain
Immunocytochemistry/ Immunofluorescence-Synaptotagmin antibody [ASV30](ab13259)
](/ps/datasheet/images/13/ab13259/Synaptotagmin-Primary-antibodies-ab13259-2.jpg)
ICC/IF image of ab13259 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13259, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Synaptotagmin antibody [ASV30] (ab13259)
This product has been referenced in:
- Wong FK & Stanley EF Rab3a interacting molecule (RIM) and the tethering of pre-synaptic transmitter release site-associated CaV2.2 calcium channels. J Neurochem 112:463-73 (2010). WB, IP; Chicken.Read more (PubMed: 19878533) »
- Zhu LQ et al. GSK-3beta inhibits presynaptic vesicle exocytosis by phosphorylating P/Q-type calcium channel and interrupting SNARE complex formation. J Neurosci 30:3624-33 (2010). WB; Rat.Read more (PubMed: 20219996) »
See all 2 publications for this product
Publishing research using ab13259? Please let us know so that we can cite the reference in this datasheet
![Synaptotagmin antibody [ASV30] for WB in Rat (13259)](/ps/Reviews/Images/ab8634_37789.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"