Anti-Syndecan 4 antibody (ab74139)
Key features and details
- Rabbit polyclonal to Syndecan 4
- Suitable for: ELISA, WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Syndecan 4 antibody
See all Syndecan 4 primary antibodies -
Description
Rabbit polyclonal to Syndecan 4 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic non-phosphopeptide from human Syndecan 4 around the phosphorylation site of serine 179 (E-G-SP-Y-D).
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Positive control
- Human brain tissue. Extracts from HepG2 cells. HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/500 - 1/1000.
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
1/500 - 1/1000. |
Target
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Function
Cell surface proteoglycan that bears heparan sulfate. -
Tissue specificity
Expressed in epithelial and fibroblastic cells. -
Sequence similarities
Belongs to the syndecan proteoglycan family. -
Post-translational
modificationsShedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3. -
Cellular localization
Membrane. Secreted. Shedding of the ectodomain produces a soluble form. - Information by UniProt
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Database links
- Entrez Gene: 6385 Human
- Entrez Gene: 20971 Mouse
- Entrez Gene: 24771 Rat
- SwissProt: P31431 Human
- SwissProt: O35988 Mouse
- SwissProt: P34901 Rat
- Unigene: 632267 Human
- Unigene: 3815 Mouse
see all -
Alternative names
- Amphiglycan antibody
- MGC22217 antibody
- OTTHUMP00000031788 antibody
see all
Images
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All lanes : Anti-Syndecan 4 antibody (ab74139) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells
Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 22 kDa
Observed band size: 22 kDa -
Immunohistochemistry analysis of human brain tissue using ab74139, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
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Immunofluorescence analysis of HeLa cells using ab74139, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab74139 has been referenced in 6 publications.
- Cao C et al. Cholesterol-induced LRP3 downregulation promotes cartilage degeneration in osteoarthritis by targeting Syndecan-4. Nat Commun 13:7139 (2022). PubMed: 36414669
- Wu Y et al. LncRNA WDFY3-AS2 promotes cisplatin resistance and the cancer stem cell in ovarian cancer by regulating hsa-miR-139-5p/SDC4 axis. Cancer Cell Int 21:284 (2021). PubMed: 34051810
- Hu X et al. Syndecan-4 promotes vascular beds formation in tissue engineered liver via thrombospondin 1. Bioengineered 11:1313-1324 (2020). PubMed: 33251971
- Chen LL et al. SDC4 Gene Silencing Favors Human Papillary Thyroid Carcinoma Cell Apoptosis and Inhibits Epithelial Mesenchymal Transition via Wnt/ß-Catenin Pathway. Mol Cells 41:853-867 (2018). PubMed: 30165731
- Ranzato E et al. Epithelial mesenchymal transition traits in honey-driven keratinocyte wound healing: comparison among different honeys. Wound Repair Regen 20:778-85 (2012). PubMed: 22882448
- Ranzato E et al. Platelet lysate modulates MMP-2 and MMP-9 expression, matrix deposition and cell-to-matrix adhesion in keratinocytes and fibroblasts. Exp Dermatol 20:308-13 (2011). PubMed: 20955204