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Overview

  • Product nameSyndecan Human ELISA KitSee all Syndecan kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    overall 6.2%
    Inter-assay
    Sample n Mean SD CV%
    1 6 197ng/ml 21 10.5%
    2 6 88ng/ml 8 9.5%
    3 6 199ng/ml 19 9.4%
    4 6 88ng/ml 9 10%
    5 6 274ng/ml 27 9.9%
    6 6 162ng/ml 19 12%
  • Tests
    2 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    4.94 ng/ml
  • Range
    8 ng/ml - 256 ng/ml
  • Recovery

    101 %

  • Assay time
    1h 45m
  • Species reactivity
    Reacts with: Human
  • Product overview

    The Human Syndecan ELISA is to be used for the in-vitro quantitative determination of soluble Syndecan-1 molecule (Syndecan) in human serum, plasma, buffered solutions or cell culture medium. This kit has been configured for research use only and is not to be used in diagnostic procedures.

    Principal:

    The Syndecan Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for Syndecan has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Syndecan concentrations, specimens and unknowns are pipetted into these wells. During the first incubation, the Syndecan antigen and a biotinylated monoclonal antibody specific for Syndecan are simultaneously incubated. After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Syndecan present in the samples.

     

    Recognizes natural and human Syndecan1

    Range: 8 ng/ml - 256 ng/ml

    Sensitivity <2.56 ng/ml

  • Notes

    Safety Information:

    • For research use only.

    • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.

    • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.

    • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.

    • Do not pipette by mouth.

    • The stop solution in this kit contains 9.8% sulphuric acid.

  • Tested applicationsELISA more details

Properties

  • RelevanceSyndecan is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. Syndecan functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered Syndecan expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein.
  • Cellular localizationCell Membrane; single-pass type I membrane protein
    • Alternative names
        CD138 CD138 antigen SDC
        SYND1 Syndecan 1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab46507 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Syndecan Human ELISA Kit images

      • ELISA - Human Syndecan ELISA Kit (ab46507)
        ELISA - Human Syndecan ELISA Kit (ab46507)
        Typical Syndecan standard curve ranging from 8 to 256 ng/mL.

      References for Syndecan Human ELISA Kit (ab46507)

      This product has been referenced in:

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"